INTRODUCTIONMedicines of plant origin have been used as a form of therapy for the relief of pain since ancient time 1 . In management of pain, the presently used drugs are well known for having side and toxic effects are either narcotics, if used, e.g., opioids or non-narcotics e.g., salicylates and corticosteroids e.g., hydrocortisone. Introducing a new synthetic product as drug, approximately 3000-4000 compounds are to be synthesized, screened and tested and hence cost of development goes up from 0.5-5.0 million dollars. Thus these synthetic drugs are very expensive. Plants represent a large number of untapped source of structurally novel compounds that might serve as lead for the development of novel drugs 2 .To overcome these problems many workers have aimed at knowing the analgesic activity of different medicinal plants and using them for the treatment of pain management as possible alternatives to chemically synthetic drugs [3][4][5][6][7] .Parkia timoriana (DC.) Merr. is a deciduous, mediumsized tree up to 50 m tall. The leaves are arranged alternate. The primary rachis is a stalk of 18-42 cm long and the pinnate leaf comes with 14-31 pairs of leaflet. It is distributed in India, Bangladesh, Myanmar, Thailand and throughout Malaysia (except New Guinea), including the Philippines 8 .Parkia timoriana are considered beneficial in the treatment of hepatalgia, oedema, nephritis, diabetes and colic, probably as a result of their diuretic and relaxing activity. They are also used as an anthelmintic. The leaves are used against jaundice 9 .

Objective: To evaluate the anti-microbial activity of ethanolic extract of fruits of Annona reticulata (family-Annonaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of fruits of Annona reticulata (family-Annonaceae) was prepared by Soxhlet extraction and analysed for phytochemical constituents using standard methods. The anti-microbial activity of the plant extract was examined against bacterial strains and fungal strains using disc diffusion method.Results: The present investigation shows the phytochemical analysis, anti-microbial activity of the ethanolic extract of the plant Annona reticulata. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, steroids, proteins and amino acids and tannins. The anti-microbial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of fruits of the Annona reticulata contain high presence of phytochemicals. The ethanolic extract of the plant was found to possess promising anti-microbial activity when compared with the standards.

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Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

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