Paraovarian cysts constitute 10-20% of all adnexal masses and are usually less than two centimeter in diameter. Authors reported a 20-year-old unmarried female with vague abdominal discomfort since 1 year. On examination abdomen was uniformly distended with central position of umbilicus. Ultrasonography showed a large cystic mass extending from pelvis to pancreas likely of ovarian origin. Biochemical evaluation was not suggestive of malignant nature of this cystic mass. So, with due care of cosmesis, surgery was planned. After the midline vertical incision, peritoneal washings were collected for oicytological analysis to further exclude malignancy. Then 5mm cannula with trocar was inserted into the cyst to suck out the fluid then shrunken cystic wall was exteriorised. Right ovary was absolutely healthy. Enucleation of the cyst was done with preservation of ovary. Histopathology reported as a papillary serous cyst adenofibroma. Follow up period was uneventful.

Intrauterine contraceptive device is important part of family planning services in India. These can be inserted post menstrually, post abortal, post-delivery or in post puerperal period. Associated complications include bleeding, pain, infection, expulsion and most serious complication as perforation of uterine wall and migration to adjacent organs. We present a case of successful laparoscopic retrieval of a misplaced cuT. A 30-year-old female para 2 live 1 presented in SDMH outpatient department with right sided lower abdominal pain since one year. NCCT scan of pelvis showed IUCD in pelvic cavity anteriorly just above urinary bladder. Laparoscopic removal of cuT was done along with tubal recanalization.

Aims: The aim of the present study is to isolate and characterize root nodulating bacteria from Cicer arientinum L cv. GNG 1958 and assess its plant growth promoting properties. The study is based on previous reports that native symbiotic bacteria may be replaced by better performing strains by corss-inoculation to increase productivity and tolarance to Biotic and abiotic stresses. Study Design:  Isolation of symbiotic bacteria, its biochemical and molecular characterization and assessment of plant growth promoting property through ammonia production, phosphate solubilization and HCN production. Place and Duration of Study: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, during 2019-2022. Methodology: Root nodulating bacterial strain was isolated from C. arientinum L. cv. GNG 1958 grown at the agricultural fields' located at the Krishi Vigyan Kendra, Banasthali Vidypapith, Rajasthan. Morphological characteristics of the isolates were noted following Bergey’s Manual of Systemic Bacteriology and biochemical tests performed as per standard methods. Molecular characterization of the potential symbiont was performed by PCR of 16s rRNA genes and the PCR product was sequenced. Plant growth promoting property was determined through ammonia production, phosphate solubilization and HCN production. Results: Root nodulating bacterial strain was isolated from C. arientinum L.cv. GNG 1958. Out of eighteen isolates, BVCA-58 grew fast and  changed the color of BTB dye in media from green to yellow and deep yellow within 2 days of incubation. White colonies were observed when BVCA-58 was inoculated on YEMA media supplemented with Congo red. BVCA-58 has the ability to produce hydrogen cyanide, ammonia and solubilize phosphate. Molecular study of BVCA-58 using 16f-16r and 63f-1244r primers showed 99.89% and 99.62% of similarity with Mesorhizobium jarvisii ATCC-33669 and Mesorhizobium erdmanii USDA-3471 respectively on performing NCBI Blast. BVCA-58 was submitted to NCBI portal as Mesorhizobium sp. strain BVCA-58 with accession nos. OP646813 and OP646810. Conclusion: The present study revealed that isolated strain from Cicer arientinum GNG 1958 variety was Mesorhizobium sp. which has potent plant growth promoting traits.