This research describes the design and determination of the biomimetic biosensor used for histamine detection in cheese and canned tuna samples. Histamine imprinted nanoparticles (MIP) were synthesized and characterized by scanning electron microscopy, and size analysis. After, histamine imprinted nanoparticles were coated onto an SPR chip surface. The modified chips were characterized by using contact angle, atomic force microscopy, ellipsometry, and scanning electron microscopy.The optic analysis by the SPR biosensor has been implemented with a PBS solution of pH 7.4. The novel biosensor shows high sensitivity, low detection limit (0.58 ng.mL À 1 ) and a wide linear range from 0.001 to 10 μg.mL À 1 . The histamine imprinted biosensor was successfully analyzed on spiked food samples. The simple procedure, easy preparation, and low production cost provide that sensors coated with biomimetic nanoparticles to be attractive for sensitive and selective detection of targets.