Today, patients and health professionals are turning to innovative approaches due to the lack of success in treating chronic wounds. This study aimed to investigate the effect of a mixture of Hypericum perforatum histopathologically, Calendula officinalis, and Aloe vera plant extracts on incisional wound healing. A total of 14 rats, 7 rats, were used in each group. The groups were divided into experimental and control. While daily wound care was performed with an herbal extract mixture in the experimental group, sterile saline was used for daily wound care in the control group. During the study, wound surface area measurement, macroscopic evaluation, and histopathological examination were performed in both groups. According to research findings, the control group's wound-healing process is shorter. Histopathological analyses revealed that wound healing was completed on day 21.