Objective: This study investigates the potential estrogenic activity of the ethanolic extract of Flemingia vestita Benth tubers using ovariectomized rat model. Materials and Methods: The ethanolic extract of F. vestita tubers has been standardized using validated HPLC method in terms of its genistein content (8.43 ± 0.05 mg/g of extract). Three to four week old young albino Wistar female rats were ovariectomized and treated for 14 days post ovariectomy with the standardized ethanolic extract at three different dose levels (100, 250, 500 mg/kg body weight) with a positive control of Estradiol valerate (1 mg/kg/day). The parameters evaluated were uterine weight, uterine glycogen, G6PDH, LDH, 17β-estradiol, progesterone, total cholesterol, triglycerides, HDL and histo architecture of uterus. Results: Treatment with the ethanolic extract of F. vestita tubers showed dose dependent increase in uterine weight, glycogen levels, G6PDH levels, estrogen and progesterone levels when compared with the ovariectomized control. Amongst three dose levels, high dose of plant extract showed significant increase in the uterine weight (p < 0.001), uterine glycogen content (p < 0.001), 17-β estradiol and progesterone levels (p < 0.001), G6PDH and LDH levels (p < 0.001) as well as significant decrease in HDL and triglycerides levels (p < 0.001) compared to ovariectomized control. Histopathological evaluation of uteri sections revealed that the high dose of the plant show increase in the endometrial response as indicated by proliferation of endometrial glands and luminal epithelium of the ovariectomized rats. Conclusion: Thus, these data suggests that ethanolic extract (500 mg/kg body weight) of F. vestita tubers may exhibit good estrogenic activity in ovariectomized rat model.