Limited knowledge of native marine biodiversity hinders effective biodiversity management to safeguard South and Southeast Asia’s marine coastal environment against the threat of invasive species transfer through shipping. In particular, sessile marine biofouling organisms in South East Asian ports are poorly known. Through the support of the ASEAN-India Cooperation Project on the Extent of Transfer of Alien Invasive Organisms in South/South East Asia Region by Shipping, a coordinated effort to examine diversity of biofouling organisms in major port areas in Southeast Asia and India was made using polyvinylchloride (PVC) panels as recruitment surfaces in a static immersion study for a period of 12 months. Not surprisingly, the study revealed that fouling patterns differed between ports possibly as a result of dissimilar hydrographic conditions. However, there were also underlying similarities that reflected a regional uniformity in the composition of fouling communities. At the same time, the alien Caribbean bivalve Mytilopsis sallei was detected in Manila Bay (Philippines), Songkhla Port (Thailand) and Singapore. This is a first simultaneous biofouling survey involving scientists and government stakeholders from India and ASEAN nations of Brunei Darussalam, Indonesia, Lao PDR, Malaysia, Myanmar, Singapore, Thailand, Philippines and Vietnam.

The fouling organisms at Songkhla Port were investigated from November 2011 to December 2012. Samples were collected using PVC panels (10 cm x 20 cm) submerged for one-month and three-month periods. Analysis of fouling panels was carried out using PhotoGrid software. In addition to slime and silt, three types of fouling organisms, namely encrusting bryozoa, barnacles and calcareous polychaetes, were observed on the submerged PVC panels. Calcareous polychaetes and molluscs were the most diverse groups (ten species) on panels that were submerged for one-month and three-month periods. Barnacles (Balanus spp.) were most abundant on panels submerged for three-month periods, while calcareous polychaetes dominated one-month panels. The dominant species of mollusc was the mussel Brachidontes sp. The major polychaetes identified were Ficopomatus macrodon, F. enigmaticus and Hydroides norvegicus. A highlight of this study was the first record of the Caribbean tubeworm Hydroides sanctaecrucis in Thailand, which has previously invaded and established in Australian waters through hull fouling.

Rapid and species-specific detection, and quantification of pathogenic bacteria are fundamental for monitoring and assessment of the risk they pose to any ecosystem. The study employed Vibrio cholerae, a human pathogen responsible for the life-threatening diarrhoeal disease, cholera and one among the most unwanted from marine bioinvasion point of view. The present study coupled fluorescence in situ hybridization (FISH) technique, a powerful tool in molecular phylogenetic discrimination, with flow cytometry (FCM), a technique used for rapid and accurate quantification of both viable but non-cultivable and non-viable microorganisms. The FISH-FCM technique was used for the first time to quantify V. cholerae (includes cultivable and non-cultivable) from different geographic regions of Southeast Asia (Brunei, Indonesia, Lao PDR, Myanmar, Philippines, Singapore, Thailand, Vietnam) and India (Goa, west coast of India). The data acquired from the analyses provides a snap shot view of the total bacterial abundance with special reference to V. cholerae. As the method developed, it was evaluated with bacterial samples collected from different sites in Southeast Asia and India, and the application of this technique to different geographical regions appears feasible. Considering that the continuous growth of the shipping industry and ballast water as one of the primary vectors responsible for the global transport of pathogenic microorganisms, the risk they present needs immediate attention. This technique will be useful in the quick and accurate detection of specific pathogens. It may also provide significant insights to quarantine measures for Ballast Water Management.

Marine phytoplankton was investigated in ballast water of ships from 2010 to 2012 with a collection of 30 marine vessels that docked at Laem Chabang International Port in Chonburi Province, Thailand. The results showed that the dominant group of phytoplankton was diatoms. The amount of phytoplankton in the ballast tanks averaged less than 10 cells/ mL, which is less than Regulation D-2 of the Ballast Water Management Convention which requires that marine organisms between the sizes of 10 ? X <50 µm should be less than 10 cells/mL and size ? 50 µm should be less than 10 cell/m3 in ballast water. Alien species of phytoplankton was not recorded in this survey.