The objective of the study was to assess; the level of knowledge, attitude and practice on cervical cancer screening (CCS) among female university students (FUS) in Ishaka, western Uganda. The study was a descriptive cross-sectional study among FUS (n = 407) in western Uganda conducted after the first nationwide lockdown in Uganda. Information was collected using a questionnaire and descriptively presented as frequency and percentages. Majority of our respondents were medical students 283 (69.5%), below 25years 339 (83.3%), with Anglican Christian religious background 150 (36.9%). More respondents above the age of 25years (p = 0.0052) and those in the medical profession (p < 0.001) had more knowledge. More medical students had a better attitude (p = 0.0043) and favorable practices (0.0134) compared to their non-medical counterparts. There is a weak correlation between attitude (r = 0.206, p < 0.001) and practice (r = 0.181, p = 0.0003) with knowledge on CCS. Observation from the present study suggests the need for more efforts in the fight against cervical cancer and encouragement of positive attitude and practice towards cervical cancer screening and uptake of vaccination.