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BACKGROUND: Needle pricks injury (NPI) is the most frequent occupational hazard for nurses and other health care workers (HCWs). It is the commonest route by which blood-borne viruses and/ or infections transmit from patients to healthcare workers. Good knowledge and safe injection practices are important concept for ANM’s. AIM AND OBJECTIVE: (1) To assess the level of knowledge on needle prick injury among ANM’s. (2) To assess level of preventive practice on needle prick injury among ANM’s. (3) To find out the association between knowledge and preventive practice of needle prick injury with selected demographic variable. MATERIALS AND METHODS: Study adopts quantitative approach and Non-experimental descriptive exploratory design was used to assess the knowledge and preventive practice of ANMs regarding needle prick injury. The population for the study was registered ANM’s working at sub center level in Etawah district. Probability simple random (lottery method) sampling technique is adopted. RESULT AND DISCUSSION: Study observed that knowledge regarding needle prick injury among ANM’s had a wide range i.e. 40% ANM had good knowledge, 50% ANM had average knowledge. ANM’s Practice observation finds out that majority (40%) ANM were belonging to good practice and 46.66% were belonging to average practice. Study find out that that association of knowledge level in ANM worker is significant with age and work experience (p<0.05). Association of preventive practice level in ANM worker was significant with duration of course (p<0.05).

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