Purpose: This study was done to investigate knowledge of intravenous practice for infection prevention and compliance, empowerment, and organizational culture awareness regarding infection control, and to identify influencing effects regarding compliance with intravenous practice for infection prevention among nurses working in small and medium-sized hospitals.Methods: A cross-sectional descriptive survey was conducted with a sample of 136 nurses working in small and medium-sized hospitals. Data were collected using self-administered questionnaires. For data analysis, descriptive statistics, independent t-tests, one-way ANOVA, Pearson correlations and a stepwise multiple regression were performed using IBM SPSS 23.0.Results: There was a significant correlation between compliance with intravenous practice for infection prevention and knowledge (r=.34, <i>p</i><.001), and between compliance with intravenous practice for infection prevention and organizational culture awareness of infection control (r=.41, <i>p</i><.001). Moreover, the factors influencing compliance with intravenous practice for infection prevention were organizational culture awareness of infection control (β=.35, p<.001) and knowledge of intravenous practice for infection prevention (β=.27, <i>p</i>=.001). These variables explained 22.7% of compliance with intravenous practice for infection prevention.Conclusion: It is necessary to an organizational culture that emphasizes infection prevention through regular monitoring and feedback and to develop regular education and training programs based on current guidelines.

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