Objectives: This study aims to verify the effect of the use of a toothpaste containing 0.5% zinc citrate and 1,450 ppm fluoride on oral malodor. Methods: In this study, the experimental group using the experimental toothpaste and the control group using the standard toothpaste control toothpaste were allowed to use each toothpaste for 4 weeks and then a clinical test to measure the change in bad breath was conducted. A study was conducted to evaluate the reduction effect. Efficacy was evaluated by conducting BB checker test and Oralchroma test three times in total before the testing 2 weeks and 4 weeks after the test. Results: As a result of measuring the degree of bad breath using the BB checker, before the start of the test, the experimental group was 46.3 and the control group was 47.2. After 2 weeks of the test, the experimental group was 38.5 and the control group was 44.2 and after 4 weeks the experimental group was 29.6 and the control group was 39.2. There was a significant difference between the experimental group and the control group after 4 weeks of the test (P<0.05). The volatile sulfur compounds measured by Oralchroma were 0.825 ng/10 ml for the experimental group and 0.819 ng/10 ml for the control group after 2 weeks of the test, and they were 0.705 ng/10 ml for the experimental group and 0.860 ng/10ml for the control group after 4 weeks of the test. It was confirmed that a significant index change appeared after 4 weeks of the test compared to before the test (P<0.05). Conclusions: It was confirmed that an improved effect of removing bad breath can be expected when a toothpaste containing 0.5% zinc citrate and 1,450 ppm fluoride is used.

Objective: This study was to investigate and compare the antifungal activity of commercial denture cleansers sold in Korea against C. albicans biofilms and to analyze changes induced in the physical properties of denture base acrylic resin, by denture cleansers.Methods: A specimen of denture base material was fabricated using polymethylmethacrylate acid for the formation of C. albicans and physical analysis. C. albicans biofilm was formed on the denture base acrylic resins using Ham's F-12 medium in a 5% CO2 incubator. The biofilms were treated with denture cleansers such as ClinicDent, DENTFIX, Polident Ⓡ , and Good Habit according to the manufacturer's instructions, and the suspension of C. albicans was inoculated on an agar plate after disrupting the biofilm. The denture base acrylic resins were treated with denture cleansers for 90 days, and surface roughness, surface hardness, and color change were analyzed using an atomic force microscope, Vickers microhardness device, and colorimeter, respectively. Results: PolidentⓇ and Good Habit showed the strongest antifungal activity against C. albicans biofilm, and DENTFIX showed the weakest antifungal activity against the biofilm, compared to the others. The denture cleansers used in this study significantly increased the surface roughness of the denture base acrylic resins (p＜0.05). Polident Ⓡ and Good Habit-treated resins showed significantly less surface hardness compared to that of the control (p＜0.05). Conclusion:No significant differences were observed in terms of physical changes among the denture cleansers-treated denture base acrylic resins. Polident Ⓡ and Good Habit had stronger antifungal activity against C. albicans biofilm than the other cleansers. Based on the results, Polident Ⓡ and Good Habit are suitable for use among denture wearers.

Objectives:The aims of this study were to investigated and compared antifungal activity of commercial denture cleansers sold in Korea against C. albicans biofilm and to analyze change of physical properties of denture base acrylic resin by the denture cleansers. Methods: The specimen of denture base material was fabricated using polymethylmethacrylate acid according to the manufacturer's protocols to use formation of C. albicans and physical analysis. C. albicans biofilm was formed on the denture base acrylic resin using Ham's F-12 medium in 5% CO2 incubator. The biofilms were treated with denture cleansers such as ClinicDent, DENTFIX, Polident Ⓡ , and Good habit according to manufacturer's instructions, and the suspension of C. albicans was inoculated on agar plate after disrupting the biofilm. The colonies were counted after incubating the plate. The denture base acrylic resin was treated with the denture cleansers for 90 days and analyzed surface roughness, surface hardness, and color change by atomic force microscope, Vickers micro hardness, and colorimeter, respectively. Results: PolidentⓇ and Good habit showed the strongest antifungal activity against C. albicans biofilm, and DENTFIX has the weakest antifungal activity against the biofilm compared to the others. The denture cleansers used in this study significantly increased the surface roughness of the denture base acrylic resin (p<0.05). In case of surface hardness, Polident Ⓡ and Good habit significantly reduced more compared to control as tap water (p<0.05). Conclusion:The significant difference of physical change among the denture cleansers treated the denture base acrylic resins did not show, and Polident Ⓡ and Good habit has strong antifungal activity against C. albicans biofilm than the others. Based on the results, Polident Ⓡ and Good habit may be suitable for use of denture wearers.