An important and perhaps critical clue to the mechanism driving the explosion
of massive stars as supernovae is provided by the accumulating evidence for
asymmetry in the explosion. Indirect evidence comes from high pulsar
velocities, associations of supernovae with long-soft gamma-ray bursts, and
asymmetries in late-time emission-line profiles. Spectropolarimetry provides a
direct probe of young supernova geometry, with higher polarization generally
indicating a greater departure from spherical symmetry. Large polarizations
have been measured for 'stripped-envelope' (that is, type Ic) supernovae, which
confirms their non-spherical morphology; but the explosions of massive stars
with intact hydrogen envelopes (type II-P supernovae) have shown only weak
polarizations at the early times observed. Here we report multi-epoch
spectropolarimetry of a classic type II-P supernova that reveals the abrupt
appearance of significant polarization when the inner core is first exposed in
the thinning ejecta (~90 days after explosion). We infer a departure from
spherical symmetry of at least 30 per cent for the inner ejecta. Combined with
earlier results, this suggests that a strongly non-spherical explosion may be a
generic feature of core-collapse supernovae of all types, where the asphericity
in type II-P supernovae is cloaked at early times by the massive, opaque,
hydrogen envelope.Comment: Accepted for publication by Nature (results embargoed until 23 March
2006); 14 pages, 2 figure
Although ␣-synuclein is the main structural component of the insoluble filaments that form Lewy bodies in Parkinson disease (PD), its physiological function and exact role in neuronal death remain poorly understood. In the present study, we examined the possible functional relationship between ␣-synuclein and several forms of matrix metalloproteinases (MMPs) in the human dopaminergic neuroblastoma (SK-N-BE) cell line. When SK-N-BE cells were transiently transfected with ␣-synuclein, it was secreted into the extracellular culture media, concomitantly with a significant decrease in cell viability. Also the addition of nitric oxide-generating compounds to the cells caused the secreted ␣-synuclein to be digested, producing a small fragment whose size was similar to that of the fragment generated during the incubation of ␣-synuclein with various MMPs in vitro. Among several forms of MMPs, ␣-synuclein was cleaved most efficiently by MMP-3, and MALDI-TOF mass spectra analysis showed that ␣-synuclein is cleaved from its C-terminal end with at least four cleavage sites within the non-A component of AD amyloid sequence. Compared with the intact form, the protein aggregation of ␣-synuclein was remarkably facilitated in the presence of the proteolytic fragments, and the fragment-induced aggregates showed more toxic effect on cell viability. Moreover, the levels of MMP-3 were also found to be increased significantly in the rat PD brain model produced by the cerebral injection of 6-hydroxydopamine into the substantia nigra. The present study suggests that the extracellularly secreted ␣-synuclein could be processed via the activation of MMP-3 in a selective manner.
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