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Organic fertilizer as an alternative to reduce the scarcity of chemical fertilizers, otherwise it can improve soil fertility. PT. Semen Gresik - Semen Indonesia Persero (Tbk.) Has a lot of plants in the treatment period in ex-mining land that needs fertilizer to help plant growth. In addition, in post-mining land, there is a lot of organic waste in the form of dry leaves under the stands of reclamation trees. The organic waste has the potential to be processed and used as organic fertilizer. This study aims to determine the utilize leaf litter in the reclaimed mine land as organic fertilizer with EM 4 and Molasses bioactivators. This study used is a laboratory experimental method. The results showed the composition of solid material in the form of leaf litter, bran, cow dung and husk charcoal in a ratio of 2: 1: 1: 1 as well as looking material in the form of molasses and EM4 with a dose of 100ml per 100kg of solid material dissolved in enough water (fertilizer can be clenched and expanded slowly and not broken). Conclusion leaf litter in the former reclaimed land can be processed and used as organic fertilizer.

<p class="5abstrak">Science is a science that learns about life in the world from all aspects. To be able to study these subjects requires a deeper understanding of the material. Application of learning innovations needs to be done to achieve the objectives of science subjects especially by teachers so as to provide hands-on experience for students to acquire innovative learning. Based on the observations at SMP Negeri 1 Bancar known that the results obtained by students less satisfactory. The process of teaching and learning is done by lecture method, when the learning process takes place many students are sleepy and chatting. To improve the learning outcomes in science subjects especially biology required the existence of learning innovation, namely by using Natural Picture learning media. The purpose of this study is to determine whether there is an influence of Natural Picture learning media on student learning outcomes in science biology subjects in SMP Negeri 1 bancar. The type of research used is Quasi Eksperimen. This research was conducted at SMP Negeri 1 Bancar with research sample that is student of class VII A which amounted to 32 students as experiment class and student of class VII E which amounted to 32 students as control class. The research design used was Pre-Test research design - Post-Test Control Group Design. Based on the results of data analysis of learning results, the average value in the experimental class given treatment using Natural Picture learning media is higher than the control class using the lecture method. The experiment class is 87.12 and the control class is 74.37. In the hypothesis test the Sig value. (2-tailed) of 0.000, when compared with α 0.05 then Sig &lt;α then H0 is rejected and H1 accepted. Thus, it can be concluded that there is a difference in mean values of experimental class learning outcomes and control classes. That is, there is the effect of learning media Natural Picture on student learning outcomes.</p><p class="6keyword"> </p>

This study aimed to analyzed the haplotype network and constructed the phylogenetic tree topology of hepatitis C virus (HCV) in Tuban, Indonesia and those from other countries, to determine the visualize genealogical relationship and inference about gene connected. The HCV isolates were collected from blood transfusion center of Tuban, East Java, Indonesia in 2015 and as a comparison, also the sequences of HCV isolates were retrieved from the GenBank®, National Center of Biotechnology Information (NCBI), USA database. To constructed of distribution map was performed through median joining analysis using Haplotype Network v4.6, whereas to constructed and phylogenetic model analyses were constructed based on the NS5B and 5’UTR regions using MEGA X, maximum-likelihood method based on the Tamura-Nei model. In the HCV NS5B region haplotype network showed high haplotype diversity (Hd=1.00) in 22 haplotypes. Based on phylogenetic analysis 3 sample isolates Tuban (BDT-55-p23, BDT-79-p23 and BDT-112-p23) were identified one group and closely related to isolates from Indonesia. However, isolates Tuban also have the same sequence characters as the isolates from Thailand. It was also confirmed in haplotype network that the three isolates are connected to each other. The identification of HCV genotypes circulating in blood donors in the Tuban of East Java confirmed were closely related to HCV isolates from Indonesia and Thailand. Therefore, this result might contribute in a better medical management towards HCV.