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Directional stress is an effective measure to evolve community structure and improve bioactivity of pit mud (PM). In this study, adding fortified Daqu in artificial PM (APM) was to disturb the microbial community and affect the metabolites furthermore. To evaluate the effect of fortified Daqu on culturing APM, microbial communities of APMs with/without adding fortified Daqu were investigated by fluorescence in situ hybridization and Illumina Miseq. These results indicated that microbes (Clostridium sp., Clostridium kluyveri, hydrogenotrophic methanogens, and acetotrophic methanogens) related to the production of key aroma compounds increased notably when fortified Daqu was added. Especially the hydrogenotrophic and acetotrophic methanogens increased by 5.19- and 4.63-fold after 30-days’ culture. Then metabolites (organic acids, volatile compounds) were also analyzed by HPLC and HS-SPME-GC-MS. Results showed that the content of butyric acid and hexanoic acid was significantly higher when adding fortified Daqu. What’s more, the proportion of esters and phenols were higher compared with the APM without adding fortified Daqu as well. The microbial compositions of APMs with/without adding fortified Daqu were observed in this study, which indicated the microbial community evolving in functional community in favor of liquor-brewing and suggested a novelty process was developed by disturbing the community diversity.

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Dissociative identity disorder (DID) is often presented in movies with the impression of danger, and its patients constantly acted as madness and crazy. With the rise of the internet, movies have become an essential communication channel, exerting a significant impact on people's understanding of the outside world. Thus, some scholars believe that movies may negatively influence people's impressions of DID since its figures have o ften been distorted. However, they failed to analyze in a more up-to-date and rigorous setting. Therefore, this study examined the connection between movies and DID, using questionnaires, movie analysis, and experiment in a more objective way. The paper concludes that DID is portrayed in distorted and biased ways in popular movies, and it influences the audiences to form and worsen the negative impressions toward DID and its patients, proving the expected hypothesis. Specifically, through movie analysis, the study found that movies still dramatize and associate the DID characters with violent behaviors, despite the movies give out some correct information in terms of the symptoms for DID. Moreover, by looking at the differences between pre-and post-screening surveys, people's impression of DID is generally negative. The willingness to get in touch with DID patients has gone down after watching DID-related movies. However, because the study only specifically examined nine participants in Dalton Academy, it is still a crucial task to extend such related research in a greater context, including a more diverse and generalizable population, further helping to understand and find ways to improve the social environment for DID patients and other mental disorders.