Understanding how plasmonic nanoparticles collectively generate heat upon exposure to light and thus increase the local temperature of the surrounding medium is critical for many applications such as plasmon-assisted microfluidics, plasmonic tweezers, and photothermal cancer therapy. Reliable temperature manipulation requires the capability to spatially and dynamically analyze local temperature profiles as a function of nanoparticle concentration and laser intensity. In this work, we present a novel method for visualization of local temperature increase using elastin-like polypeptides (ELP). We also propose a robust algorithm that allows the construction of reliable calibration curves using known boundary conditions and Boltzmann sigmoid fit applied to the ELP solution's temperature-absorption transfer function. Using this technique, for the first time, we successfully demonstrated how surface and volume distribution of the nano-heaters affect collective heat generation. This approach allows the visualization of dynamic 2D-temperature profiles and simultaneously enables the measurement of specific temperature at any point in a 2D-map. The experimental setup is compatible with conventional optical microscopy and requires no specialized hardware or complex sample preparation. Finally, the real time visualization of plasmonic heating offers an opportunity to control outcomes of thermo-plasmonics which enables a myriad of practical applications.
Analysis of individual cells at the subcellular level is important for understanding diseases and accelerating drug discovery. Nanoscale endoscopes allow minimally invasive probing of individual cell interiors. Several such instruments have been presented previously, but they are either too complex to fabricate or require sophisticated external detectors because of low signal collection efficiency. Here we present a nanoendoscope that can locally excite fluorescence in labelled cell organelles and collect the emitted signal for spectral analysis. Finite Difference Time Domain (FDTD) simulations have shown that with an optimized nanoendoscope taper profile, the light emission and collection was localized within ~100 nm. This allows signal detection to be used for nano-photonic sensing of the proximity of fluorophores. Upon insertion into the individual organelles of living cells, the nanoendoscope was fabricated and resultant fluorescent signals collected. This included the signal collection from the nucleus of Acridine orange labelled human fibroblast cells, the nucleus of Hoechst stained live liver cells and the mitochondria of MitoTracker Red labelled MDA-MB-231 cells. The endoscope was also inserted into a live organism, the yellow fluorescent protein producing nematode Caenorhabditis elegans, and a fluorescent signal was collected. To our knowledge this is the first demonstration of in vivo, local fluorescence signal collection on the sub-organelle level.
As part of the Deepwater Horizon toxicity testing program, a number of laboratories generated oil slicks in the laboratory to study potential toxic effects of these oil slicks on aquatic organisms. Understanding the details of how these slicks affect aquatic organisms requires careful correlation between slick thickness and the observed detrimental effects. Estimating oil film thickness on water can be challenging since the traditional color-based technique used in the field is very imprecise. Also, as we demonstrate here, the films formed on the water surface are highly nonuniform on a microscale level, and thus uniform thin film thickness measurement techniques based on optical interference do not work. In this paper, we present a method that estimates the local thickness of weathered oil slicks formed on artificial seawater using light transmission and Beer-Lambert's law. Here, we demonstrate results of careful calibration together with the actual thickness estimation. Due to the heterogeneity of the slicks formed, we present slick thickness as a range of thicknesses collected from multiple points within the oil slick. In all the experiments we used oil samples provided by the Natural Resource Damage Assessment toxicity testing program for the Deepwater Horizon oil spill. Therefore, this study has an important practical value and successfully addresses unique challenges related to measurements involving complex, viscous, paste-like heterogeneous substances such as weathered crude oil.
Here we present a new design and FDTD simulations of light delivery by a nanowire-based intracellular endoscope. Nanowires can be used for minimally invasive and very local light delivery inside cells. One of the main challenges is coupling of light into the nanowire. We propose a new plasmonic coupler interface between cleaved optical fiber and a nanowire, and optimize light coupling efficiency and contrast.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.