ABSTRAKTerdapat indikasi bahwa kesadaran peternak ayam ras petelur terhadap biosekuriti masih rendah. Makalah ini bertujuan untuk menentukan tingkat adopsi peternak ayam ras petelur di Sulawesi Selatan dalam berbagai tindakan biosekuriti. Kabupaten Sidenreng Rappang (Sidrap) dipilih sebagai lokasi penelitian karena terkenal sebagai pusat peternakan ayam ras petelur. Jumlah sampel adalah 60 responden. Sampel dipilih secara acak dari dua kecamatan yang paling banyak peternaknya yaitu Baranti dan Maritengae. Data dikumpulkan menggunakan kuesioner terstruktur dan wawancara. Data ditabulasi dan dianalisis menggunakan metode skoring status biosekuriti. Status biosekuriti digunakan untuk mengetahui tingkat adopsi biosekuriti. Status biosekuriti diperoleh berdasarkan penerapan tindakan biosekuriti yang terdiri dari 9 tahap yaitu: input peternakan, lalu lintas ke peternakan, jarak dari sumber penyakit dengan kandang, keadaan peternakan, biosekuriti pada pagar peternakan, biosekuriti antara pagar dan kandang, biosekuriti di pintu kandang, lalu lintas dalam kandang dan kerentanan terhadap penyakit. Berdasarkan indeks adopsi, hasil penelitian menunjukan bahwa adopsi biosekuriti pada peternak ayam ras petelur di Sulawesi Selatan diklasifikasikan sebagai parsial adopter.Kata kunci: adopsi biosekuriti, peternak, ayam ras petelur ABSTRACTIt was indicated that layer smallholders awareness of biosecurity was low. This paper aimed to determine the level of adoption within the South Sulawesi layer smallholders of a range of standard biosecurity measures. Sidenreng Rappang (Sidrap) regency was chosen as a location of the research, because it was famous as a central of layer smallholders. Total sample was 60 respondents. The sample was chosen through random sampling from two districts which were the most populous of layer smallholders, namely Baranti and Maritengae. Data were collected using structured questionnaires and depth-interview. The data were tabulated and analysed using a simple method of scoring with regard to their biosecurity status. The status of biosecurity was used to know the level of biosecurity adoption. Biosecurity status was obtained based on the adoption of biosecurity measures which consisted of 9 stages: farm inputs, traffic onto farms, distance from sources of pathogens to shed, exposure of farm, biosecurity at farm boundary, biosecurity between farm boundary and shed, biosecurity at the shed door, traffic into the shed and susceptibility of the flock. Using adoption index, this research revealed that biosecurity adoption of layer smallholders in South Sulawesi was classified into a "partial adopter".