Penelitian ini bertujuan untuk menganalisis karakteristik morfometrik ayam kampung yang disukai dan tidak disukai di Desa Tirtomulyo, Kecamatan Plantungan, Kabupaten Kendal, Jawa Tengah. Materi menggunakan 105 ekor ayam kampung yang terdiri dari 5 ekor jantan dan 100 ekor betina. 30 peternak sebagai responden untuk menentukan ayam disukai dan tidak disukai. Data dianalisis menggunakanujit, Principal Component Analysis dengan menggunakan alatbantuStatistical Analysis System (SAS) University Edition. Hasil penelitian memperlihatkan bahwa ayam jantan memiliki perbedaan yang nyata (P<0,05) pada lingkar tarsometatarsus dan panjang sternum, sedangkan ayam betina memiliki perbedaan nyata (P<0,05) pada bobot badan dan tinggi jengger. Parameter pembeda pada ayam kampung jantan dan betina yang disukai dan tidak disukai adalah panjang sayap yaitu 0,619 dan 0,922. Peta penyebaran ayam jantan dan betina yang disukai dan tidak disukai mempunyai ukuran tubuh yang lebih besar dibandingkan ayam jantan dan betina yang tidak disukai.
Sutiyono, Sutopo, Ondho YS, Setiatin ET, Samsudewa D, Suryawijaya A, Lestari DA, Kurnianto E. 2018. Genetic diversity ofOngole Grade Cattle of Rembang District, Central Java, Indonesia, based on blood protein polymorphism. Biodiversitas 19: 1429-1433. The objective of this study was to identify genetic diversity in Ongole Grade cattle of Rembang District, Central Java, Indonesia,based on blood-protein polymorphism. A total of 34 blood samples were collected from unrelated Ongole Grade cattle and the bloodplasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide GelElectrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of four loci, namely Albumin (Alb), Postalbumin(Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed the presence of polymorphism in all of the four bloodproteinloci, average heterozigosity value (H) being 0.506 and almost all of the blood-protein loci studied showed deviation from Hardy-Weimberg Equilibrium (HWE). In conclusion, the study indicated that population of Ongole Grade cattle in Rembang, Indonesia hashigh genetic diversity.
The aim of this research was to determine the female reproductive disorders in cattle reared by local farmers in the Distric of Kaliori, Rembang Regency, Central Java Province. A total of 94 cattle were used, in which had minimal one of each incisors had been replaced. The study used survey methods, and data were collected by interviewing with ranchers, rectal palpation, identification of the incisors, and body condition score of the cattle. In the implementation of the study, cattle were taken to a place determined by the chairman of the group of farmers (field or home page). The parameters of study were unheard of oestrus or not, the amount of artificial insemination, the number of incisors changed, body condition score of each cattle, the feed given, and their maintenance. The data were analyzed using statistical descriptive analysis on the mode, range, and percentage. The results showed that of the 94 cattle, which have disorders of reproductive activity as much as 80. Samples with impaired reproductive activity were divided into three groups. The first group was the old heifers that had no oestrus 25.00%, the second group was cattle that were more than three times applied artificial insemination and had not been pregnant 45.00%, and the third group was cattle that more than three months after the last giving birth had no oestrus 30.00%. The other reproductive disorders that occured in individual of the cattle was inactive ovaries (follicle undeveloped) 2.50%, 6.25% ovary hypofunction, ovarian cystic 1.25%, endometritis 2.50% and 2.50% abnormal uterus. In conclusion, the largest reproductive disorders in cattle caused by nutritional factors that provided by the farmers, and small disturbances due to some diseases and abnormal reproductive organs.
Abstract. Setyorini YW, Kurnianto E, Sutopo, Sutiyono. 2023. Identification polymorphism of LHR and FSHR genes in Indonesian Holstein dairy cattle associated with productive and reproductive traits. Biodiversitas 24: 2898-2905. Luteinizing and follicle-stimulating hormones and their receptors play an important role in the reproductive system’s hormonal activity and physiological function. This study aimed to identify the genotype variation of the Luteinizing Hormone Receptor (LHR) gene exon 11 and the Follicle-stimulating Hormone Receptor (FSHR) gene exon 10 and to elucidate the associating between polymorphism with the milk and reproductive traits in dairy cattle. A total of 100 samples of deoxyribonucleic acid (DNA) from Indonesian Holstein dairy cattle were used in this study. Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) was performed for genotyping of the Single Nucleotide Polymorphism (SNP) of the LHR and FSHR genes with the HhaI and AluI restriction enzymes, respectively. The collected phenotype data were milk production, quality, and reproductive trait. In addition, the general linear model procedure in the SAS program was carried out to investigate the association of genotypes with productive and reproductive traits. The results showed that the LHR gene in the population of this study was monomorphic, while the FSHR gene was polymorphic with three genotypes, namely CC, CG, and GG. The sequences of the FSHR gene indicated the presence of a mutation at nucleotide number 2037 that substitutes cytosine for guanine. Cows with the CC genotype showed better parameter service per conception (P<0.05) than the other genotypes but did not correlate with other reproductive parameters and milk traits. Therefore, it was concluded that SNP g.2037C>G polymorphism at FSHR gene exon 10 was identified in the Indonesian Holstein Dairy cattle population and associated with service per conception parameters but not with milk trait.
Penelitian ini bertujuan untuk mengidentifikasi faktor-faktor pendukung dan faktor penghambat pembentukan profil pelajar Pancasila di SD Negeri Deresan. Selain itu, dirumuskan strategi SWOT untuk meningkatkan pembentukkan Profil Pelajar Pancasila. Metode yang digunakan yaitu kualitatif. Adapun analisis dilakukan secara deskriptif. Data yang digunakan berupa data primer hasil wawancara dengan guru SD Negeri Deresan dan data sekunder melalui analisis kajian hasil penelitian sebelumnya mengenai faktor-faktor pendukung dan faktor-faktor penghambat implementasi penguatan pendidikan karakter dalam upaya pembentukan profil pelajar Pancasila. Hasil penelitian menunjukkan bahwa pembentukkan profil pelajar Pancasila di SD Negeri Deresan terdapat faktor-faktor pendukung internal diantaranya terdapat mata pelajaran PPKn dan Pembiasaan bersalaman yang menjadi alternatif awal pembentukkan profil pekajar Pancasila. Sedangkan faktor pendukung ekternal yaitu dengan adanya kurikulum 2013 menjadi basis awal untuk melaksanakan penguatan karakter pada peserta didik. Faktor-faktor penghambat internal meliputi terbatasnya kemampuan guru untuk menginterpretasikan mengenai profil pelajar Pancasila, kurangnya kemandirian guru untuk belajar secara mandiri melalui website yang telah disediakan. Adapun faktor-faktor penghambat meliputi belum adanya sosialisasi dan belum adanya bimtek yang spesifik mengenai pembentukan profil pelajar Pancasila, dan belum tersedianya modul dan kondisi pandemi yang menyulitkan pembentukkan profil pelajar Pancasila. Strategi yang ditawarkan yaitu mulai dari pembuatan modul integratif dari pemerintah pusat dan pemerintah daerah. Rencana mengambil dua parameter dari profil pelajar Pancasila yaitu kebhinnekaan dan kemandirian (kewirausahaan) yang diintegrasikan dalam kegiatan sekolah. Kebhinnekaan diintegrasikan dalam kegiatan ta’ziah atau melayat bersama siswa untuk membentuk sosio-tolerance dan kewirausahaan diimplementasikan melalui kegiatan membatik yang dipamerkan dan diperjual-beliakan dalam even-even peringatan hari besar di SD Negeri Deresan.This study aims to identify the supporting and inhibiting factors for the formation of the Pancasila student profile at SD Negeri Deresan. In addition, a SWOT strategy was formulated to increase the building of the Pancasila Student Profile. The method used is qualitative. The analysis was carried out descriptively. The data used in the form of primary data from interviews with SD Negeri Deresan teachers and secondary data through an analysis of the results of previous studies regarding the supporting factors and inhibiting factors for the implementation of strengthening character education in an effort to form a profile of Pancasila students. The results showed that the formation of the profile of Pancasila students at SD Negeri Deresan contained internal supporting factors including PPKn subjects and the habit of shaking hands which became the initial alternative for forming the profile of Pancasila students. While external supporting factors, namely the existence of the 2013 curriculum, became the initial basis for carrying out character strengthening in students. Internal inhibiting factors include the limited ability of teachers to interpret the profile of Pancasila students, the lack of teacher independence to study independently through the website that has been provided. The inhibiting factors include the absence of socialization and the absence of specific guidance and technology regarding the formation of a Pancasila student profile, and the unavailability of modules and pandemic conditions that make it difficult to form a Pancasila student profile. The strategy offered is starting from making integrative modules from the central government and local governments. The plan takes two parameters from the Pancasila student profile, namely diversity and independence (entrepreneurship) which are integrated into school activities. Diversity is integrated in ta'ziah activities or mourning with students to form socio-tolerance and entrepreneurship is implemented through batik activities which are exhibited and traded in big day commemoration events at SD Negeri Deresan.
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