Background: Fenugreek (Trigonella foenum-graecum) a widespread aromatic herb, holds an important place in Indian traditional medicine system. A fenugreek or methi seed commonly used to flavour food contains various alkaloids, different saponins and fixed oil content. The steroidal nature of natural saponins has high market potential as alternative to Dioscore species, the current declining resource of commercial saponins. The current experiment designed to induce and identify somaclone variants of fenugreek resistant to powdery mildew, rich in saponins and fixed oil content with acceptable yield.Methods: Seeds of fenugreek var. Gujarat Methi-2 (GM-2) were used for in vitro regeneration during 2019 laboratory experiment. Explants cultured on Murashige and Skoog (MS) media supplemented with different concentrations of 2,4-D and NAA. The induced calluses were further transferred in to the regenerated semi solid MS medium with various levels of BAP for the development of calliclones. For root induction, shoots were transferred in White’s rooting media with different concentrations of Indole Acetic Acid.Result: Our experimental findings shows induction and detection of somaclonal variants, as a possible source of genetic improvement in fenugreek with limited information on genetic diversity. This approach could generate new genotypes useful to pharmaceutical industries with competitive yield and resistance to powdery mildew.

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