The aim of this research is to establish proper external and internal conditions for conducting pedagogical activity that reveal the value basis of professional education. The research justifies the need to analyse the guidelines of a person’s professional self-identity, contents of professional activity motivation, peculiarities of maintaining the mental and physical health of a person, and the capacity of an educational institution’s organisational culture. A number of indicators of value mechanisms of a teacher’s professional longevity is revealed. A diagnostic program of the project, aimed at studying the value content of a teacher’s behaviour strategies during the process of professional activity, was designed. The content of the hypothesis is connected with the assumption of value-semantic inter-conditionality of professional longevity. The research revealed significant interrelations of professional longevity with some psychological factors of value-semantic nature. Analysis of the organisational culture of an educational institution as a factor providing (preventing) the professional longevity of a teacher was carried out. The dominant tendency of describing the interconnection between an educational institution’s organisational culture and the level of emotional burnout syndrome of teachers working within certain cultural models is revealed.