Magnetic sedimentation processes near ferromagnetic wire were applied for the cellular and the biotechnological monitoring. Numerous biological applications ofmagnetic sedimentation, or high gradient magnetic separation (HGMS), achieved mainly for human blood cells, have shown that it is possible to distinguish between the diamagnetic Fe-zerospin oxidized states and the paramagnetic Fe-high-spin reduced ones in red blood cells. The precision methods of the measurements ofcapture travelling (magnetic diffusion) time or accumulation (magnetic sedimentation) radius in HGMS have shown that it is really to determine the weak dia-or paramagnetic shifts ofmagnetic susceptibility up to 0.7 10'°(SI). The capture and sedimentation ofparticles with 5-40 .tm sizes on single magnetized wires were investigated using microscopic video-recording type. Magnetic susceptibility bars for cells in normal and disturbed states demonsrated the high reproducibility and narrow distribution patterns.
Magnetophoretic and optical methods combined with pulsating weak magnetic field 1-10 Hz were applied to observe resonant vibrations of biological microparticles with anisotropic magnetic properties. Modeling of oxidative disorders in tissues was realized using reactive oxygen species (ROS) initiation and ferritin incorporation into porous sorbent beads and blood cells. Microscopic video recording of cells in high gradient magnetic separation allowed to determinate changes of magnetic moments under stress activating influences.
The speckle pulse-analyser is used to measure the human resonant mechanovibrations under pulsating magnetic influences, to conduct their Fourier-spectra analysis and dynamical processes documentation. The fast coherent response oforganism on super weak ultra-low-frequency magnetic field was recorded on coherent cell's vibrations. Possible biophysical mechanism are suggested and proved on experiments with blood cells. In particularly, gradient steady magnetic fields could reveal the vibrational potency ofcells in pulsating magnetic fieldsprovided ofthe appearance of cellular magnetic sign-diffence and anisotropy under pathological situations in organism. From the disturbance's oxidative process in cells which give the paramagnetic shift ofcell's magnetic susceptibility relatively the normal state, the single cells or certain parts oforgans become more paramagnetic than other native tissue. Arising magnetic vectors of living systems appear oscillations in pulsating magnetic fields ( 1-6 Hz, 1-100 nT).
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