Abstract. The study presents an updated database on the impact of the main technological factors on the content of pollen grains and quality indicators of natural honey: diastase number, hydroxymethylfurfural content and invertase activity. The purpose of the research was to update the database on the impact of the main technological factors on the microscopy and quality indicators of natural honey: diastase number, hydroxymethylfurfural content and invertase activity. Based on the goal, the following tasks were solved: On the basis of the laboratory of the FSC of Beekeeping, production samples of honey were heated at 75 °C for 10 minutes, followed by natural cooling, and filtered through a filter with a cell size of 0.15 mm; they were stored in the production room for a year with a temperature fluctuation in the range from –1 °C to 32 °C. The relevance of the study is to study the main indicator of the quality of honey – invertase activity, changes in the value under the influence of technological and zootechnical factors, with its subsequent introduction into the state standard for natural honey. The scientific novelty of the study lies in the fact that for the first time the values of invertase activity indicators, the content of hydroxymethylfurfural after heating and filtration of honey were obtained. Methods. The study of the indicators was carried out in accordance with the methods of the state standard GOST 19792-2017. As a result, it was found that heating honey at 75 °C for 10 minutes followed by natural cooling contributes to a decrease in diastase activity by an average of 69.7 ± 6.43 %, a decrease in invertase by an average of 81.0 ± 2.37 %, and an increase in the content of GMF by an average of 79.1 ± 1.74 %. Honey filtration reduces diastase by an average of 11.1 ± 1.47 %, and invertase activity by an average of 11.3 ± 2.34 %. Storage of honey for a year with a temperature fluctuation from –1 °C to 32 °C contributes to a decrease in the activity of diastase by 51.3 ± 5.27 %, invertase by 61.0 ± 1.91 % and an increase in the content of hydroxymethylfurfural by an average of 82.9 ± 1.80 %.