Respiratory tract nerves have cell bodies outside (sensory, sympathetic) and inside (parasympathetic) the organ and contain bioactive peptides. These include calcitonin gene-related peptide and tachykinins (sensory nerves), vasoactive intestinal polypeptide (parasympathetic nerves), and neuropeptide with tyrosine (sympathetic nerves). Because transplantation interrupts the extrinsic nerve supply to the tissues, we have examined transplanted human respiratory tracts (n = 11) removed at retransplantation 2 to 42 months after the primary transplant in order to determine whether any nerves and peptide synthesis persist. As controls to establish nerve distribution in human respiratory tract, tissues were obtained from 10 lung resections and five autopsies. Cryostat sections were immunostained to demonstrate the general neural marker PGP 9.5, neuropeptides, and the catecholamine-synthesizing enzyme tyrosine hydroxylase. Nerves immunoreactive for PGP 9.5 were detected in all transplanted tissues. They were fewer in number overall than in control tissue, significantly so in epithelium of trachea and bronchus where they were present sparsely in only three cases. Nerves immunoreactive for tyrosine hydroxylase were significantly fewer in the transplants. Peptide-immunoreactive nerves were also reduced in number in the transplants, except for vasoactive intestinal polypeptide, which was only significantly changed in blood vessels in the lung. Ganglion cells immunoreactive for tyrosine hydroxylase and neuropeptide with tyrosine were seen in the transplanted tissues in five cases, but never in the control tissues. We conclude that whereas some nerves and neuropeptide synthesis persist after extrinsic pulmonary denervation, potentially significant changes also occur, including the appearance in intrinsic parasympathetic neurones of immunoreactivity for a catecholamine-synthesizing enzyme and a peptide normally found in sympathetic nerves.

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In some applications such as field stations, disaster situations or similar conditions, it is desirable to have a contactless, rugged means to collect fingerprint information. The approach described in this paper enables acceleration of the capture process by eliminating an otherwise system and finger cleanup procedure, minimizes the chance of the spread of disease or contaminations, and uses an innovative optical system able to provide rolled equivalent fingerprint information desirable for reliable 2D matching against existing databases. The approach described captures highresolution fingerprints and 3D information simultaneously using a single camera. Liquid crystal polarization rotators combined with birefringent elements provide the focus shift and a depth from focus algorithm extracts the 3D data. This imaging technique does not involve any moving parts, thus reducing cost and complexity of the system as well as increasing its robustness. Data collection is expected to take less than 100 milliseconds, capturing all four-finger images simultaneously to avoid sequencing errors. This paper describes the various options considered for contactless fingerprint capture, and why the particular approach was ultimately chosen.

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