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This book covers most of the topics with latest information on stem cell in general and mesenchymal stem cell (MSC) research in particular. The book is divided into 17 chapters covering almost all aspect of stem cell technology including definition of different types of stem cells, isolation of MSC from different animals, culture and characteristics of mesenchymal stem cell, osteoinduction potential of MSC, protocol for isolation of bone marrow derived MSC, fat derived MSC, embryonic stem cells (ESC), protocol for MSC passaging. This book also includes different research findings on application of bioengineered stem cell technologies in skin wound healing, burn wound healing, critical bone defect healing, large bone defect healing, corneal wound healing and in liver regeneration. The main objective of this book is to provide the latest information to meet the requirements of not only undergraduate and post graduates research scholars but also to the teachers, biologists and clinician involved in animal treatment and research. With more than 150 good quality colour photographs of stem cell isolation, stem cell culture, stem cell morphology, stem cell characteristics and stem cell application in different surgical interventions.