Introduction: There are expected to have become 1.9 million new cancer diagnoses and 609,360 cancer - related deaths in 2022, or about 1,670 mortality each day. Regardless of human development level, throughout the world, the disease is a leading source of morbidity and mortality. Cancer like disorders have been documented also in Indian subcontinent since antiquity. In the 19th century, the very first cancer cases were diagnosed, and the burden of cancer began to climb in the 20th century, when current treatment facilities were severely inadequate. Roudra Rasa is a special Ayurvedic preparation for Arbuda (Cancer) mentioned in Bhaishajya Ratnavali. It has already been tested clinically in Oropharengeal cancer, with Ayurvedic treatment incorporating Roudra Rasa showing a greater benefit. Cancer cell lines of human are useful models for exploring cancer biology and testing the treatment effectiveness of anti - cancer drugs in the lab. That’s why Michigan Cancer Foundation-7 (MCF-7 - Breast), Human Colorectal Adenocarcinoma Cell Line (HT-29 - Colon), Human Lung Adenocarcinoma (HOP-62), Lung cancer epthelial cell (A549 - Lung), and cell line from prostate carcinoma (DU145), prostate cancer cell line (PC-3 - Prostate), these cancer cell lines have chosen to examine Roudra Rasa's anti cancer activity. Aim and Objectives: Pharmaceutico analytical study of Roudra Rasa and evaluation of its Anti-carcinogenic And acute toxicity studies in selective cell lines and albino Wistar rats respectively. Materials and Methods: Roudra Rasa will be prepared by traditional method and quality control parameters will be assessed. Anti-carcinogenic activity will be carried out in selected cancer cell lines. and acute toxicity testing will be done in accordance with OECD guidelines 423. Observations and Results: Observations of pharmaceutical, analytical and experimental studies will be noted and presented in the forms of table, charts, photographs etc. Conclusion: If Roudra Rasa shows any significant Anti-Carcenogenic activity then it would be safe and efficient medicine for Cancer.

Ayurveda is an ancient science of life that uses a wide range of formulations or herbal compounds to treat various diseases. Each drug must be scientifically, pre-clinically, and clinically evaluated in order to gain global acceptance. As part of the safety evaluation process, regulatory authorities around the world require data on new drug genotoxic potential.MusalyadiChurna(MSDC) is a classical Ayurvedic formulation in herbal powder dosage form consisting of eight ingredients of herbal origin and indicatedin the treatment infertility. This herbal combination may be useful, however mixture of substances can be dangerous and genotoxic, causing harm to living cells' genetic material and resulting in mutations that induce serious issues for the host, such as cancer and birth defects; consequently, genotoxicity testing is essential.The study aimed to evaluate the genotoxic, antigenotoxic and protective properties of MusalyadiChurnaagainst the bone marrow of albino mice. MusalyadiChurna's genotoxicity were assessed using the "Mammalian Bone Marrow Chromosome Aberration assay," and the classical powder's antigenotoxic and protective activities against cyclophosphamide-induced genotoxicity in mice were also assessed using the same assay.Mice were administered MusalyadiChurna orally for four weeks at a dose of 25 mg/25 g mouse. Cyclophosphamide was utilized as a mutagen, and a single dose of 50 mg/kg b.w. was given intraperitoneally (i.p.) 24 hours after the final dose of MusalyadiChurna in the pre-treatment regimen and 24 hours before the first dose of MusalyadiChurna in the post-treatment regimen.The chromosomal aberration assay revealed that the herbal compound formulation MusalyadiChurnaat 1000 mg/Kg body wt. had no genotoxic activity against the bone marrow of Swiss albino mice. Using the same experiment, the formulation demonstrated both protective and antigenotoxic properties against Cyclophosphamide-induced mutagenicity in mice.Further research should be to capitalize on MusalyadiChurna’s potential as an alternative source of natural compounds to treat genotoxic problems.