Urinary tract infections (UTIs) are one of the most common human bacterial infections. While UTIs are commonly associated with colonization by Escherichia coli , members of this species also have been found within the bladder of individuals with no lower urinary tract symptoms (no LUTS), also known as asymptomatic bacteriuria. Prior studies have found that both uropathogenic E. coli (UPEC) strains and E. coli isolates that are not associated with UTIs encode for virulence factors. Thus, the reason(s) why E. coli sometimes causes UTI-like symptoms remain(s) elusive. In this study, the genomes of 66 E. coli isolates from adult female bladders were sequenced. These isolates were collected from four cohorts, including women: (1) without lower urinary tract symptoms, (2) overactive bladder symptoms, (3) urgency urinary incontinence, and (4) a clinical diagnosis of UTI. Comparative genomic analyses were conducted, including core and accessory genome analyses, virulence and motility gene analyses, and antibiotic resistance prediction and testing. We found that the genomic content of these 66 E. coli isolates does not correspond with the participant’s symptom status. We thus looked beyond the E. coli genomes to the composition of the entire urobiome and found that the presence of E. coli alone was not sufficient to distinguish between the urobiomes of individuals with UTI and those with no LUTS. Because E. coli presence, abundance, and genomic content appear to be weak predictors of UTI status, we hypothesize that UTI symptoms associated with detection of E. coli are more likely the result of urobiome composition.

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Prophages play a critical role in the evolution of their host species and can also contribute to the virulence and fitness of pathogenic species. Here, we conducted a comprehensive investigation of prophage sequences from 5,383 publicly available Pseudomonas aeruginosa genomes from human as well as environmental isolates.

To better understand the potential effects of climate change on atmospheric dynamics, this paper studies Rossby wave breaking and isentropic stratosphere‐troposphere exchange (STE) in the Northern Hemisphere between 320 and 380 K during 1981–2015 using the Modern‐Era Retrospective Analysis for Research and Application version 2 data. The isentropic STE is estimated using Contour Advection. Our results show that anticyclonic wave breaking events have become more frequent, especially in summer at higher isentropic surfaces, and cyclonic wave breaking events have become less frequent at 320 K. The anticyclonic wave breaking has shifted poleward in summer. The isentropic STE is found to be strongest over the regions where Rossby wave breaking activities are most frequent. Both isentropic STE and Rossby wave breaking are shown to be strongest in summer and weakest in winter. Our results do not show any discernable trends during isentropic STE during 1981–2015.

Klebsiella variicola is an opportunistic pathogen in humans. It also has been associated with bovine mastitis, which can have significant economic effects.