Hypospadias is considered as second most common birth defect in children with 1 in every 250 male births worldwide and 1 in every 150 boys in India. Urethroplasty is only mode of hypospadias repair which is associated with wide range of complications like urethrocutaneous fistula, meatal stenosis, narrow urethra. Rising incidence of these complications demands judicious postoperative care and urethral calibration. Objective: To develop and operationalize protocol on urethral calibration for children undergone urethroplasty among caregivers and nursing personnel. Method: An operational study was conducted on caregivers and nursing personnel at pediatric surgery OPD. Total of 40 study subjects i.e. 32 caregivers and 8 nursing personnel included by total enumeration sampling. Study was conducted in three phases. In 1st phase, current practices of urethral calibration were assessed.10 observations were taken to identify lacunae regarding procedure. Protocol on urethral calibration was developed by extensive literature review, expert opinions using Delphi technique. In 2nd phase, training sessions were conducted using lecture cum discussion, demonstration and return demonstrations, booklets and posters. In 3rd phase, assessment of effectiveness of urethral calibration was done by evaluating occurrence of complications related to urethroplasty. Results: Significant (p < 0.001) increase in mean performance score of urethral calibration of caregivers and nursing personnel was achieved up to 22.75 ±1.10 and 23.50 ± 0.92 after four and three subsequent return demonstrations respectively. Conclusion: Protocol on urethral calibration is effective in developing skills among caregivers and nursing personnel related to procedure and improving surgical outcome of urethroplasty among children.