We study the effect of chargeable monomers on the conformation of dendrimers of low generation by computer simulations, employing bare Coulomb interactions. The presence of the latter leads to an increase in size of the dendrimer due to a combined effect of electrostatic repulsion and the presence of counterions within the dendrimer, and also enhances a shell-like structure for the monomers of different generations. In the resulting structures the bond-length between monomers, especially near the center, will increase to facilitate a more effective usage of space in the outer-regions of the dendrimer.
STAT3 is an important transcription factor involved in immunity and cancer. In response to cytokine stimulation, STAT3 becomes phosphorylated on a single tyrosine residue. Tyrosine-phosphorylated STAT3 accumulates in the nucleus, binds to specific DNA response elements and induces gene expression. Unphosphorylated, latent STAT3 shuttles constitutively between cytoplasm and nucleus. We analysed the importance of previously identified putative nuclear localization sequences (NLS) and nuclear export sequences (NES) for nucleocytoplasmic shuttling of latent STAT3 using STAT3-deficient cells reconstituted with fluorescently labelled STAT3 mutants. Mutation of a putative NLS or NES sequence did not impair nucleocytoplasmic shuttling of latent STAT3. We were also interested in the structural requirements for dimerization of unphosphorylated STAT3 and its relevance for nucleocytoplasmic shuttling. By native gel electrophoresis and dual-focus fluorescence correlation spectroscopy (2f-FCS) we identified the N-terminal domain (amino acids 1–125) to be essential for formation of unphosphorylated STAT3 dimers but not for assembly of tyrosine-phosphorylated STAT3 dimers. In resting cells, the monomeric N-terminal deletion mutant (STAT3-ΔNT) shuttles faster between the cytoplasm and nucleus than the wild-type STAT3, indicating that dimer formation is not required for nucleocytoplasmic shuttling of latent STAT3. STAT3-ΔNT becomes phosphorylated and dimerizes in response to interleukin-6 stimulation but, surprisingly, does not accumulate in the nucleus. These results highlight the importance of the N-terminal domain in the formation of unphosphorylated STAT3 dimers and nuclear accumulation of STAT3 upon phosphorylation.
Thermosensitive composite hydrogels that consist of a poly(acrylamide) hydrogel matrix with embedded micrometer-sized poly(N-isopropylacrylamide) microgel beads are promising models for complex, heterogeneous gels. We investigate the coupling of the microgel beads with the gel matrix and the formation of interpenetrating networks inside the microgels by confocal two-focus fluorescence correlation spectroscopy (2fFCS). This technique serves to study the effects of the heterogeneous structure of the composite hydrogels on the diffusive mobility of nanoscopic dextran tracers within the gels. Our investigations reveal that the formation of interpenetrating networks inside the embedded microgel beads depends on their cross-link density: whereas interpenetrating networks are formed inside weakly cross-linked beads, they are not formed inside strongly cross-linked beads. If the formation of interpenetrating networks occurs, the temperature-dependent swelling and deswelling of the beads is obstructed. In addition, the mobility of dextran tracers inside the embedded microgel beads is hindered compared to those in free beads and in the surrounding gel matrix. Surprisingly, the surrounding poly(acrylamide) hydrogel matrix swells inhomogeneously when the embedded poly(N-isopropylacrylamide) beads collapse upon heating. This indicates the formation of pores near the surface of the collapsed beads, offering promising means to tailor composite hydrogels for applications as membranes with tunable permeability. Our experiments also demonstrate the utility of 2fFCS to study spatially resolved diffusion in complex environments, which is of great interest in biomaterials research.
Summary Background Epidermal differentiation is a multilevel process in which keratinocytes need to lose their organelles, including their mitochondria, by autophagy. Disturbed autophagy leads to thickening of the epidermis as seen in pachyonychia congenita (PC), a rare skin disease caused by mutations in keratins 6, 16 and 17. Objectives To ask if mitophagy, the selective degradation of mitochondria by autophagy, is disturbed in PC and, if so, at which stage. Methods Immortalized keratinocytes derived from patients with PC were used in fluorescence‐based and biochemical assays to dissect the different steps of mitophagy. Results PC keratinocytes accumulated old mitochondria and displayed disturbed clearance of mitochondria after mitochondrial uncoupling. However, early mitophagy steps and autophagosome formation were not affected. We observed that autolysosomes accumulate in PC and are not sufficiently recycled. Conclusions We propose an influence of keratins on autolysosomal degradation and recycling. What's already known about this topic? Terminal epidermal differentiation is a multistep process that includes the elimination of cellular components by autophagy. Autophagy‐impaired keratinocytes have been shown to result in thickening of epidermal layers. Hyperkeratosis also occurs in pachyonychia congenita (PC), a rare skin disease caused by mutations in keratins 6, 16 and 17. What does this study add? Keratins contribute to mitochondrial quality control as well as maintenance of mitochondria–endoplasmic reticulum contact sites. Keratins influence autolysosomal maturation or reformation. What is the translational message? Overaged mitochondria and autolysosomes accumulate in PC. Mutations in keratin 6a lead to severely impaired mitophagy, which might contribute to PC pathogenesis.
Our findings suggest that increased inflammasome activation may be involved in skeletal muscle pathology in ALS. Furthermore, elevated levels of NLRC4, caspase 1 and IL1β reflect early changes in the skeletal muscle and may contribute to the denervation process.
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