This study aims to utilize a sufficient amount of palm kernel cake (PKC) and buffalo rumen fluid as a source of enzymes to improve the quality of PKC that can be used in broiler feed. The study was conducted in two stages. The first stage was to test the level of buffalo rumen fluid enzyme at various doses (0.0%, 0.75%, 1.5%, 2.25%, and 3.0% (v/w) in PKC incubation to measure dry matter (DM), organic matter (OM), crude fiber (CF), and dissolved glucose total (DGT) contents as hydrolyzed PKC. The second stage was to determine the usage level of hydrolyzed PKC in broiler feed. A total of 288 DOC MB202 strains were randomly allocated to one of five treatments with four replicated cages of 12 birds in a completely randomized design. Treatments were the various level of hydrolyzed PKC as following: 0%, 6%, 12%, 18%, 24%, and 30%. The variables were feed consumption, body weight gain (BWG), feed conversion ratio (FCR), final body weight (FBW), carcass, and digestive organs weight. The level of buffalo rumen fluid enzymes had a significant effect on DM, CF, and DGT content, while it was not significant on OM. The optimum level was 2.25% and it was used in the second stage. The use of hydrolyzed PKC up to 18% was not different (P>0.05) in BWG, FCR, and FBW compared to controls. The use of hydrolyzed PKC up to 24% decreased BWG, FCR, and FBW, but feed consumption did not differ (P>0.05) compared to control. Carcass and digestive organ weight were not affected (P>0.05) by the treatment. It can be concluded that the incubated buffalo rumen fluid enzymes at a level of 2.25% could improve the quality of PKC. The use of hydrolyzed PKC could be applied up to 18% without affecting the performance of broilers

Objective: The research was conducted to calculate feed, calcium (Ca) intake, Ca requirements, and egg production for Arabic hens during the early egg-laying period. Materials and Methods: A total of 135 30-week-old Silver female Arabic pullets were randomly allocated to one of three treatments with five replicate cages with a semi-scavenging system of nine pullets per cage in a completely randomized design and allowed to choose Ca from limestone and oyster shells. As a control (T1), pullets were only given a complete feed with Ca and phosphorus percentages according to Hy-line International in 2018. Other treatment feeds were control feed without limestone fed with a combination of limestone (T2) or oyster shell (T3) separately. Results: The treatments had no effect (p > 0.05) on feed, grit and Ca intake (gm/bird/week), egg weight (gm), egg mass, egg production (%), and feed efficiency, but had an effect (p < 0.05) on Ca concentration (%). Ca concentration was the same at T1 and T3, and both were higher than at T2. Conclusion: The female Arabic chickens could fulfill Ca requirements by selecting from different sources of Ca. Limestone is better than the oyster shell as a source of Ca. The Ca requirement for Arabic hens in the early laying period based on the Ca concentration of the feed intake is sufficient at around 3.64% since producing the same number of egg production and heavier egg weight compared to a higher Ca level.