The present feeding trial was conducted to assess the optimal level of phytase supplementation required for maximum nutrient absorption and growth performance of Labeo rohita fingerlings fed canola mealbased diet. A standard diet having 30.21% protein and an energy value of 4.26 kcalg −1 was used as reference diet. The experimental diet having similar protein and caloric density was formulated by using 70% reference diet and 30% of canola meal as test ingredient. This experimental diet was then divided into seven test diets and were supplemented by graded levels (0, 250, 500, 750, 1000, 1250 and 1500 FTU kg −1) of phytase enzyme. Chromic oxide was used in reference and test diets as an inert marker. Results showed that phytase supplementation at 750 FTU kg −1 level effectively increased apparent digestibility coefficients of crude protein (64%), crude fat (76%) and gross energy (68%) as compared to reference and other phytase-supplemented diets. The results of present study showed increased growth and feed performance of fingerlings in response to phytase supplementation. Maximum performance was obtained by the fish fed on test diet having 750 FTU kg −1 level. It was concluded that 750 FTU kg −1 level of phytase supplementation in canola meal-based diet is sufficient for increasing nutrient digestibility and growth performance of L. rohita fingerlings.
BackgroundStevia (Stevia rebaudiana Bertoni) natural, safe, non-toxic, non-caloric sugar substitute is rich source of pharmacologically important glycoside stevioside that is linked to the pathology and complications of hyperlipidemia.MethodsThe present research was carried out to explore the anti-hyperlipidemic effect of aqueous extract of Stevia rebaudiana Bertoni leaves in albino rats. For this purpose, hyperlipidemia was induced by administration of Cholesterol (90% E, Appli Chem, Darmstadt, Germany) mixed at dose of 400 mg/kg body weight of rats in their daily routine feed. The hyperlipidemic rats were administered with aqueous stevia extract at different dose levels (200, 300, 400 and 500 ppm/kg b.w.) for 8 weeks; the control rats were fed basal diet during this period. Ethical approval for the current research was obtained from Institutional Review Board Faculty of Science & Technology Government College University, Faisalabad, Pakistan.ResultsStevia aqueous extract decreased the body weight gain by lowering the feed intake of hyperlipidemic rats. Furthermore, administration of stevia extract at different levels significantly (P < 0.05) lowered the TC (125.22 ± 5.91 to 110.56 ± 5.81 mg/dL), TG (102.13 ± 6.89 to 98.62 ± 7.22 mg/dL), LDL (33.02 ± 4.79 to 22.77 ± 4.36 mg/dL), VLDL (21.22 ± 5.79 to 19.33 ± 5.95 mg/dL) levels and LDL/HDL ratios (0.83 ± 1.22 to 0.54 ± 1.66 mg/dL) from H1 to H4. Conversely, it improved the HDL (39.76 ± 4.34 to l42.02 ± 4.39 mg/dL) level in hyperlipidemic rats compared with untreated rats after eight weeks study period.ConclusionIt is concluded that aqueous extract of stevia has anti-hyperlipidemic effects in albino rats, and therefore could be a promising nutraceutical therapy for the management of hyperlipidemia and its associated complications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.