The present investigation was aimed to assess the antioxidant potential of various fractions of Berberis baluchistanica Ahrendt. The organic fractions were obtained by adding distilled water in crude methanolic extract of the plant and subsequently partitioning with organic solvents namely, n-hexane, chloroform, ethyl acetate and n-butanol, respectively. Phytochemical screening showed presence of useful antioxidants in more amounts in polar fractions of this plant. The antioxidant activity of all these fractions and remaining aqueous fraction was evaluated by using five methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay ferric thiocyanate assay by Linoleic acid, total phenolic contents (TPC) and total antioxidant activity by phosphomolybdenum method. Ethyl acetate soluble fraction possessed 87.98±1.08% inhibition of DPPH radical at a concentration of 60µg/mL as compared to other fractions. The IC50 of this fraction was 15.96 ± 1.50µg/mL, relative to butylated hydroxytoluene (BHT, a reference standard), having IC50 of 12.33±0.87 µg/mL. It also have strong degree of total antioxidant activity i.e. 0.98 ± 0.07 (absorbance at 695 nm) as well as highest FRAP value (360 ± 1.4 TEµM/mL), highest total phenolic contents (143.51 ± 1.68 GAE mg/g) and highest value of inhibition of lipid peroxidation (52.496 ± 1.16% at concentration of 500 µg/mL) as compared to the other studied fractions. n-Butanol fraction also showed good results.