No abstract

The seasonal effect on haematology and body composition of Rohu and Mrigal carp were evaluated. Fifty-four fish of each species (Rohu length and weight 28.00±0.34 cm and 385±3.40 g; Mrigal carp length and weight 29.48±0.24 cm and 393±5.48 g) were collected from the Dera Ismail Khan. The values of WBCs (9.31 and 8.41×106 mm-3), RBCs (2.37 and 2.12×106 mm-3), Hb (9.58 and 8.78) and HCT (38.06 and 36.04 %) of Rohu and Mrigal carp were higher in summer whereas declined in winter (P<0.05). The MCV, MCH and MCHC of both species were higher (P<0.05) in winter, and decreased during summer. The moisture content of both Rohu and Mrigal carp was higher during winter followed by spring and lower in summer season. Lipid and protein content of both species were higher (P<0.05) in summer and lower in winter. The moisture content, proteins and lipid were found higher in Rohu than Mrigal carp. According to the findings, it was concluded that the water quality parameters vary seasonally which in turn impacts on body physiology of fish species.

Authors' Contribution SSH and SN planned the research work and wrote the manuscript. SN, IA, AK and AUR conducted the research work. RK, SUK and NN collected the samples. SK and MK analyzed the data and revised the manuscript.

The aim of the current study was to assess the effect of two different fortified feeds with different concentrations of two important medicinal plants (Withania coagulans and Zingiber officinale) on the mucosal immunity of Labeo rohita. After a dietary intervention, mucus was tested against five pathogenic bacteria (in-vitro), while experimental fish were tested against the ectoparasite (Lernaea) (in-vivo). Our results revealed that all fish groups fed with different concentrations (1, 1.5, and 2%) of Z. officinale had low molecular weight proteins and did not develop any significant signs of parasitic infection, with low mortality rate; whereas the groups that were fed with W. coagulans (particularly with 1% and 2%), including a control group, developed rapid signs of infection with high mortality rate. The highest hemagglutination titer value was recorded for the fish fed with 1% and 1.5% of Z. officinale. The lowest value was found for the fish fed with 2% of W. coagulans. The mucus of all fish of fortified groups was active and inhibited the growth of tested bacterial pathogens as compared to the control group. Further, Z. officinale groups showed greater efficacy against bacteria as compared to the W. coagulans groups. In conclusion, Z. officinale can be considered as a potential and functional ingredient in aquaculture feed. Furthermore, future studies should be conducted to investigate more details on the subject.

Assessing fish haemato‐biochemical profile and growth performance is essential to monitor the influence of fish feed and habitat on fish physiology. The influence of two regularly used different commercial feeds and varied environments such as pond and biofloc systems on haemato‐biochemical and growth parameters of Oreochromis niloticus was studied by the results of 4‐month experiment. Fish ponds and biofloc systems where all the fish were supplied either feed A and B were surveyed weekly. The crude protein content of feed A was 30%, while that of feed B was 28%. Physico‐chemical characteristics of water in all culturing systems were suitable for Nile Tilapia production throughout the study period. Results revealed a significant difference (p < 0.05) in most haemato‐biochemical parameters. Growth performance was better in fish fed on feed A than feed B in both culture systems. In between, the habitat wide range of haemato‐biochemical indices and fish growth parameters were significantly higher in biofloc technology than pond culture system. However, no significant difference (p > 0.05) was recorded in fish enzymatic activities in both systems. Furthermore, it was found that both the length and weight gain were positively associated with the crude protein of the feeds. However, in the case of biofloc, crude protein of both feeds works far better than the pond, as indicated by the p‐value and regression R square. Current study delivers the basic knowledge to observe fish blood and growth parameters, which could be essential to understanding the fish culture management and environment.