Effective chemotherapy is an essential component in the fight against malaria. Thus, in the face of the emergence and rapid spread of resistance to artemisinin derivatives, a molecule used as first-line treatment for malaria, it is important to search for new antimalarial molecules. The eradication of malaria requires the research of antimalarial drugs with gametocytocidal effects. The objective of this work was to set up cultures propagating Plasmodium falciparum gametocytes from clinical isolates and to evaluate the gametocytocidal and schizonticidal activity of Entandrophragma angolense extracts. Gametocytes were produced with asexual forms of clinical isolates in culture. The gametocytocidal activity of the extracts was evaluated by microscopy. Schizonticidal activity was evaluated using the SYBR Green method. The formation of gametocytes until maturation was observed. Gametocytocidal activity was evaluated by the percentage of gametocyte inhibition. It ranged from 95.69% to 82.76% and from 93.1% to 63.83% respectively for the hydroethanolic and methanolic extract of Entandrophragma angolense. The inhibitory concentration 50 percent of the extracts was determined. It ranges from 05.73 to 14.76 µg/mL. Generally, the extracts showed significant gametocytocidal and schizonticidal activity. This work is a precursor for the research of gametocytocidal molecules, a source of antimalarial molecules blocking malaria transmission.