No abstract

The organic phosphates analyzer, originally developed by us based on the anion-exchange separation on an Asahipak NH2P-50 column and the postcolumn phosphomolybdic acid reaction, was improved for the analysis of biological materials. The anion-exchange separation was optimized to resolve several model phosphates by using a LiCI linear gradient elution. An acetonitrile pretreatment method was devised for simple and effective deproteinization and the extraction of organic phosphates in rat heart muscle. The analyzer was successfully applied to the determination of organic phosphates in rat heart muscle.