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Abstract:We introduce a color imaging method in our digital holographic microscope system (DHM). This DHM can create color images of freely floating, or moving objects inside a large volume by simultaneously capturing three holograms using three different illumination wavelengths. In this DHM a new light source assembly is applied, where we use single mode fibers according to the corresponding wavelengths that are tightly and randomly arranged into a small array in a single FC/PC connector. This design has significant advantages over the earlier approaches, where all the used illuminations are coupled in the same fiber. It avoids the coupling losses and provides a cost effective, compact solution for multicolor coherent illumination. We explain how to determine and correct the different fiber end positions caused tilt aberration during the hologram reconstruction process. To demonstrate the performance of the device, color hologram reconstructions are presented that can achieve at least 1 µm lateral resolution.

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Using Digital Holographic Microscopy (DHM) we can gather information from a whole volume and thus we can avoid the small depth of field constraint of the conventional microscopes. This way a volume inspection system can be constructed, which is capable to find, segment, collect, and later classify those objects that flow through an inspection chamber. Digital hologram reconstruction and processing, however, require considerable computational resources. We are developing volume reconstruction and object detection algorithms that can speed up considerably by parallel hardware implementation. Therefore, we put these tasks into operation on a GPU. As data transfer of the reconstructed planes would slow down the algorithm, all the reconstruction, object detection processes are to be completed on the parallel hardware, while fine tuning of object reconstruction and classification will be done on a CPU later. The actual speed up of the GPU implemented algorithm comparing to its conventional CPU realization depends on the applied hardware devices. So far we reached a 10 times acceleration value.