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The use of biopesticides in pest management and pre-harvest disease and crop pest control have been advocated in recent years. This is because of their eco-friendliness and suitability for pest control in the agricultural industry. The objective of this study was to determine the antibacterial and pesticidal potential against sugar ant of metabolites produced by Bacillus species. The species were B. proteolyticus, B. thuringensis, B. cereus and B. subtilis. Metabolite production was carried out in batch experimental setup. The inoculated flasks were incubated in an incubator shaker for 120 h at temperature of 37 °C ± 2 °C. Metabolite extraction was carried out using the acid precipitation method. The crude metabolites were characterized using Fourier Transform Infrared (FTIR) and Gas Chromatography Mass Spectroscopy (GC-MS). Antibacterial activity of the metabolites was carried out both in agar and broth media while pesticidal potential was carried out using the diet-fed approach. All the metabolites showed antibacterial activity against the test pathogens used for investigation. This was irrespective of whether they were used singly or in combination. Generally, the rate of kill of the sugar ants by the respective metabolites was directly proportional to metabolite concentration in the diet. In the control diet setup with no added metabolite, no mortality was recorded throughout the period of incubation. The study findings gave an indication of the potential of these metabolites for possible control of phytopathogens.

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Aims: To isolate and identify bacteria with proteolytic and lipolytic properties from cow dung sample and abattoir effluent. Also, to determine the proteolytic and lipolytic activities of the bacteria isolated and carry out quantitative analysis of the enzymes produced. Place and Duration of Study: Cow dung from the rearing ground and abattoir effluent were collected into sterile conical flasks each from Ekiti State General abattoir, Ado-Ekiti in March 2017 and brought to the laboratory for analysis. Methodology: Serial dilution was carried out on the samples and 1 ml each of the required diluents was dispensed into Petri dishes and nutrient agar added for isolation of bacteria. Biochemical tests were carried out on the isolates for identification. Protease assay was carried out using UV/Visible spectrophotometer at 280 nm while lipase assay was done by simple titration method using Tween 20 as a substrate on the bacterial isolates. Statistical analysis was carried out using SPSS 20.