The aim of the study was to evaluate the effect of plasma acid on the uterine tissue of laboratory animals in vitro.Materials and methods. Treatment of dimethyl sulfoxide – water solution and water for injections with a spark discharge in air resulted in a decrease in pH, which contributed to generation of plasma acid in the solutions. We incubated uterine tissues in vitro in plasma acid at room temperature for 30 minutes. The treated tissues were examined histologically and immunohistochemically.Results. We showed that plasma acid had pronounced biological activity. Immunohistochemistry was used to show that, depending on the type of a solution, plasma acid altered generation of nitrosative damage products (3-NT) and oxidative DNA damage (8-OHdG) and modulated the number of cells with high proliferative potential (including CD133+ cells) and production of vascular endothelial growth factor (VEGF). These effects contributed to the general cytotoxicity of plasma acid solutions.Conclusion. During 30-minute exposure in vitro, plasma acid prepared from the dimethyl sulfoxide (DMSO) – water mixture exhibits various biological effects in uterine tissue samples obtained from experimental animals. Plasma-treated water exerts cytotoxic effects associated with oxidative DNA damage and promotes induction of pro-angiogenic activity in the uterine tissue. Plasma-treated DMSO does not have a cytotoxic effect. It inhibits cell proliferation, reducing the population of CD133+ cells and VEGF production in the tissue.

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The aim of the research. To study the molecular effects of water treated with spark-discharge plasma on tissues of the cyst wall of the ovarian endometrium in vitro. Material and methods. Tissues of the ovarian endometrial cyst capsule obtained during the surgical treatment of women through laparoscopic access were incubated in various modes in plasma acid, ethanol and physiological solution at room temperature. Plasma acid was produced by treating water for injection with spark-discharge plasma to a decrease in pH<2.0. Aft er the immunohistochemical study of the preparations, a comparative analysis of ablative and apoptogenic action via TUNEL was performed, as well as analysis of proangiogenic action (VEGFA), endothelial progenitor cells (CD133), protein damage (NT), DNA damage (8-OHdG) and proliferation activity (Ki67) of different treatment methods from the standpoint of potential plasma acid efficacy for sclerotherapy of ovarian endometrioma as compared to standard sclerosant: ethanol. Results. Th e study has demonstrated a more pronounced ablation and pre-apoptogenic effect of plasma acid on tissues of the ovarian endometrium capsule than ethanol. In addition, a decrease in neoangiogenesis and protein damage from active forms of nitrogen (protective action) compared to ethanol was shown. Conclusion. Water treated with spark-discharge plasma can be considered an eff ective alternative to ethanol for ovarian endometrioma sclerotherapy. The sequential action of plasma acid and ethanol increases the sclerosing action of ethanol.