Musa paradisiaca is a medicinal plant that is used widely in Nigeria for the preparation of various herbal remedies. This study investigated the effects of premature Musa paradisiaca (plantain) fruit on the reproductive hormones and characteristics of female wistar rats. Twenty female Wistar rats with regular oestrous cycle with an average weight of 145g were used. The animals were randomly grouped and divided into four groups of five animals each. Musa paradisiaca extract was administered for 14 days while monitoring the estrous cycle. Twenty hours after the last administration, animals in their proestrus were mated while animals on the oestrous phase were sacrificed, and blood samples taken. Phytochemical analysis of premature Musa paradisiaca revealed that terpenes, sterols, terpenoids, alkaloids were heavily present. The extract of premature Musa paradisiaca altered the estrous cycle of Wistar rats. Resorption was observed in high dose group. Significant increase in FSH, LH were observed in the treatment groups. Reduction in Progesterone level was observed in the high dose treatment group. In conclusion, the extract of Musa paradisiaca potentially alters fertility, inhibited implantation and had a significant effect on the reproductive hormones.