A new method of the nonsingle-Lorentz spectra analysis is described. The method is founded on the mathematically correct regularization procedure permitting one to reconstruct the smoothed distribution function of Lorentzians, contributing to the spectrum, over their widths. Influence of the noise and background as well as the role of the spectral band and resolution finiteness was investigated by use of the computer simulation. To demonstrate the possibilities of the method, the distribution functions of the latexes, vesicles and 30s subunits of E. coli ribosomes were reconstructed from scattered light spectra, measured by photon correlation spectroscopy. The regularization procedure proves to be the most reliable method for the polydispersity analysis.

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