Aim: Study of the effectiveness of in situ bacteriocin production by lactic acid bacteria (LAB) to control Listeria monocytogenes in dry-fermented sausages. Methods and Results: Two bacteriocin-producing strains: Lactococcus lactis subsp. lactis LMG21206 and Lactobacillus curvatus LBPE were grown in a pilot scale fermentor and lyophilized to be directly used in dry sausage fermentation. A commercial starter culture (Bel'meat TM SL-25) not inhibitory to L. monocytogenes (Bac ) starter) was mixed (1 : 1) with each of the two lyophilized bacteriocin-producing strains to obtain starters active against the pathogen (Bac + starter). Anti-Listeria effectiveness of the Bac + starters was studied in dry-fermented sausages. The meat batter was experimentally contaminated with a mixture of four different strains of L. monocytogenes (10 2 -10 3 CFU g )1 ). The results showed that L. monocytogenes did not grow in any of the contaminated batches, but no significant decrease (P > 0AE05) was observed either in the positive control (no added starter culture) or in samples fermented with the Bac ) starter culture during the fermentation period and up to 15 days of drying. When the Bac + starter contained Lb. curvatus LBPE, cell counts of L. monocytogenes decreased to below the detectable limit (<10 CFU g )1 ) after 4 h of fermentation and no survivors could be recovered by enrichment beyond day 8 of drying. When the Bac + starter culture containing Lc. lactis LMG21206 was used, a decrease in Listeria counts to below the detectable limit was achieved after 15 days of drying. Conclusions: The bacteriocin-producing strains studied may be used as adjunct cultures for sausage fermentations to control the occurrence and survival of L. monocytogenes. Significance and Impact of the Study: Addition of the Bac + strains, especially the Lb. curvatus strain would provide an additional hurdle to enhance the control of L. monocytogenes in fermented meat products.
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