No abstract

Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 mug/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergy syndrome might experience fewer problems consuming freshly picked apples.

conducted surveys into issues relating to the acquisition, use, management and support of Virtual Learning Environments (VLEs). A number of other studies provide information on these issues during this period. Together they provide a substantial body of evidence that allows an analysis of the factors that enhance or inhibit institutional take-up and support provision for VLEs within the UK higher education sector.There is clear evidence of increasing use of VLEs but not of widespread change in pedagogic practice. VLE management is increasingly centralised in all matters considered strategic, with dedicated devolvement occurring for a range of support activities. Differences in practice exist between old and new universities. There is in general negligible interest in standards or in institutional collaboration.