FP can be used to assess the QT prolongation and proarrhythmic potential of drug candidates; however, experimental conditions such as HPF frequency are important for obtaining reliable data.

The complete mitochondrial genome sequence of 10 walleye pollocks, Theragra chalcogramma , from the Japan Sea and Bering Sea was determined. The 16 568-16 571 bp genome contains the same 37 mitochondrial structural genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in all other vertebrates analyzed, in an organization identical to that of other bony fish. The major non-coding region had several conserved sequence features. Nucleotide variations of ND1, ND5, and control region were high, and these regions appear to be good candidates for high-resolution markers in population studies.

Abstract. The white shark, a top predator inhabiting the world's oceans, is an endangered species. However, knowledge of its life-history traits and population structure is still limited. We hypothesised that life-history traits would vary among populations because the species' various habitats are diverse and change through time. Age was estimated by counting growth bands in the centra of white sharks caught in Japan. The von Bertalanffy growth parameters were estimated at L N ¼ 455 cm TL, k ¼ 0.196 year À1 and t 0 ¼ À1.92 years for males and L N ¼ 607 cm TL, k ¼ 0.159 year À1 and t 0 ¼ À1.80 years for females. The growth rate to maturity was higher than that known for individuals from California and South Africa. Male sharks matured at 310 cm TL at 4 years of age and females began to mature at ,450 cm TL and 7 years. The D-loop-region sequences of mitochondrial DNA extracted from Japanese white sharks and GenBank datasets from sharks of California, Australia, New Zealand and South Africa indicate that Japanese white sharks form a monophyletic clade separate from the populations of other regions. The results suggest that unique life-history traits of Japanese white sharks may be caused by genetic differences.

Cells obtained from malignant lymph nodes and the peripheral blood of 106 patients with non‐Hodgkin's lymphomas were examined for T‐ and B‐cell characteristics. Surprisingly, 79 cases were of the T‐cell type on the basis of spontaneous rosette formation with sheep erythrocytes (E‐rosettes). Of the remaining cases, 15 were B‐cell in nature (monoclonal S‐Ig positive), seven were non T‐, non B‐cell and four cases were undetermined. Forty‐nine (62.0%) of the T‐cell malignancies were of a leukemic variety, characterized by pleomorphism in the peripheral blood cell size, and histological appearance. Most of the leukemic T‐cells showed obvious lymphocytic differentiation, with condensed nuclear chromatin and scant cytoplasm, although in many of the cases, the lymphomatous infiltrate was dominated by large or pleomorphic lymphoid cells. All tumors were of a diffuse variety, and on histologic examination included a mixed type (21 cases), PDLL forms (15 cases), a large lymphoid cell type (eight cases), and WDLL forms (five cases). Although the mixed type with a pleomorphic lymphoid infiltrate was distinctive, there has been considerable variation from case to case. Clinically this unusual T‐cell, leukemic variety of non‐Hodgkin's lymphomas primarily involved middle‐aged and elderly subjects, and was characterized by wide spread organ invasion (preferentially to the liver, spleen and skin), resistence to chemotherapy, and a poor prognosis. A mediastinal mass was not observed in all cases. The patients had a median survival of only ten months.

Adult C57BL/10 mice