Carbazole was metabolized by Aspergillus flavus VKM F-1024 forming few monohydroxylated products. The structure of metabolites was determined by TLC, GC, MS and 1 H NMR analyses. 3-Hydroxycarbazole was revealed as a major bioconversion product, 1-hydroxy-and 2-hydroxycarbazoles were observed as minor products. In the presence of 1-benzoylindole, the hydroxylation position shifted toward preferable accumulation of 2-hydroxycarbazole and the formation of 2,6-and 2,7-dihydroxycarbazoles. This effect and microbial formation of these metabolites have never been reported before. At the conversion of Nacetyl-and N-benzoylcarbazoles, carbazole was the major product, while 1-, 2-and 3-monohydroxycarbazoles were formed in small amounts.