Abiotic stressors such as drought are particularly detrimental to tomato vegetative growth and yield. Breeding and selection for drought-tolerant genotypes is a significant strategy for addressing this challenge. In this study, we used ten SSR primers for drought tolerance on six parents of tomato genotypes were the chosen parental genotypes included one wild type, i.e., Solanum pimpenillifolium (LA:411) and five cultivated genotypes Solanum lycopersicum, i. e., Edkawi, Super Marmande, Super Strain B, Castle Rock and Peto 86 and their fifteen crosses. Five primers were succeful and observed positive and negative markers for drought tolerance. Genetic diversity was estimated to be between 0.485 and 0.947 using SSR data, while there was very high genetic similarity (0.999) between (F 1 8 and F 1 7) (super Marmande x LA:411 and super Marmande x Edkawi) respectively. LA:411 and Edkawi could be source of drought tolerance.