Cassava roots start to deteriorate within 2-3 days after harvest when not processed. This has posed a serious challenge to both farmers and processors alike due to lack of appropriate storage techniques. Efforts made in the past to proffer solution to these problems have centered on the successful storage of cassava roots for few weeks with a little investigation on the changes in quality attributes of cassava roots during storage. Physico-chemical and sensory analysis were evaluated in this study using standard methods; as the stored cassava roots were processed into 'gari', at an interval of two weeks for six weeks. The moisture content of the stored root was affected by the relative humidity of the environment. An interaction between the storage period, methods, and physicochemical composition, except moisture content and protein, were not significant at P< 0.05 from a fresh product. Average acceptability score for 'gari' processed from cassava roots stored in a trench is higher than those produced from roots stored in moist sawdust. However, the study shows a significant difference (p<0.05) between the 'gari' processed from cassava roots stored using the
Original Research Article
Parquetina nigrescens leaves have been used in traditional medicine as an important and highly efficacious herbal remedy and have been recommended as a potential source of antimicrobial agent. Three extracts of the plant obtained using n-hexane, methanol and water were used as solvents. Phytochemical analysis of the plant extracts showed important bioactive compounds such as flavonoids, saponins, tannins, terpenes, steroids, phenols and glycosides, but alkaloids were absent in all the three extracts. Agar disk diffusion method was used to study the antimicrobial activity of the extracts at different concentrations which showed activity against three gram negative bacteria Escherichia coli, Klebsiella pneumoniae and Proteus spp., one gram positive bacteria Staphylococcus aureus, and two fungus Aspergillus flavus and Candida albicans with zone of inhibition ranging from 5-15 mm for n-hexane extract, 6-16 mm for methanol extract and 1-11 mm for aqueous extract compared to zone of inhibition for the standard antibacterial drug, 0.5 mg/mL Streptomycin that ranges from 13 – 37 mm and the zone of inhibition for the standard antifungal drug 5 mg/mL fluconazole that ranges from 24 – 25 mm. Klebsiella pneumoniae was the most inhibited while E. coli was the least inhibited by the extract, and Candida albicans was found to be resistant to the extracts in all the concentrations. TLC finger-printing of the extracts using the solvent system – butanol : acetic acid : ethanol : distilled water in the ratio 50:10:10:30 showed spots with peaks different retention times ranging from 0.24 - 0.74 cm. The results provide justification for the use of the plants in folk medicine to treat various infectious diseases.
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