Bacterial cellulose (BC) is a naturally produced as an exopolysaccharide from some bacteria. It has excellent properties over the plant cellulose and has numerous applications in many fields including food, pharmaceutical, textile, paper manufacturing and other industries. However, a major limitation of bacterial cellulose production is the high cost of carbon substrate. The study aims to reduce the cost of bacterial cellulose using a cheap carbon source. This study presents feasibility in the production of bacterial cellulose using the starchy effluent waste from the Thai fermented rice noodle manufacturing process as a low-cost substrate by Acetobacter xylinum TISTR 975. The optimizations of culture conditions for bacterial cellulose production were also investigated under static culture. The results indicated that starchy effluent waste from the Thai fermented rice noodle manufacturing process performs well for the production of bacterial cellulose by supplementing with 50 g/L sucrose and 2% olive oil under the static condition. The structure and physical properties of bacterial cellulose were characterized using SEM, FTIR and XRD. In summary, the starchy effluent waste from the Thai fermented rice noodle manufacturing process can be used to produce bacterial cellulose which is a high value-added, sustainable and green product.