Poly(a-L-glutamic acid) (PGA) was grafted with amino acid and ofigopeptide spacers up to 5 amino acids with the use of ~,~I-~arbony~diimidazo~e and 2~3-dihydro-1,2-bentisoth~azole-3~n-l~l-dioxide (saecharin~ as an additive, and these polypeptides were characterized. The antitumor antibiotic adriamycin was covalently coupled via an amide bond onto PGA and onto the grafted polymers with the use of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ); these conjugates were characterized. The conjugates containing Gly-Gly--L-Leu spacer arms did yield free adriamycin upon digestion with papain. Adriamy~in gave fairly stable complexes with PGA-~driamy~in and branched po~ypeptide-adriamycin conjugates; these complexes were characterized.

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