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This paper is a step forward by making real time control system design to monitor and control process variables to ensure its smooth operation and desired reliability. Liquid nitrogen level in the condenser is a core component of isotopic separation process to obtain (¹³C), so is needed a special focus on monitoring and control. The goal of the work is to design a controller for the liquid nitrogen in the condenser of (¹³C) isotope separation column using NI-PXI 1031 (PCI eXtensions for Instrumentation) from National Instruments. The designed ON-OFF controller is implemented in LabVIEW and are discussed the advantages and disadvantages.

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