Thirty-six multiparous Holstein cows were fed a mixture of corn silage and concentrate [1:1; dry matter (DM) basis] and long hay (0.9 kg/d) through wk 18 of lactation. Beginning at 30 d prepartum through wk 4 of lactation, the total mixed rations of 18 of these cows were top-dressed daily with 10 g of Biomate Yeast Plus (Chr. Hansen's, Inc., Milwaukee, WI). The other 18 cows served as controls. At wk 5, both control and treated cows were divided into three groups and fed 0, 10, or 20 g/d of yeast. Yeast supplementation during early lactation significantly improved DM intake, milk yield, and the digestibility of crude protein and acid detergent fiber. Least squares means for DM intake, fat-corrected milk yield, crude protein digestibility, and acid detergent fiber digestibility for cows fed 0, 10, 20 g/d of yeast during wk 5 to 18 of lactation were 23.8, 24.7, and 25.0 kg/d; 37.7, 40.7, and 41.4 kg/d; 78.5, 80.8, and 79.5%; and 54.4, 60.2, and 56.8%, respectively. Although numerical responses in DM intake and milk yield were greater for cows fed 20 g/d of yeast than for cows fed 10 g/d of yeast, the response was not significant.