The pot culture experiment was carried out to evaluate the influence of different combinations of microbial inoculants (VAM + AZO + PSB) on soil phosphatase activity andnutrients (N, P, K, Cu, Zn, Fe, Cu & Mn) of Steviarebaudia plant rhizosphere on 20th, 40th and 60th day. The study revealed that the combined application of microbial inoculants onsoil Alkaline and Acid phosphatase activity has been found maximum at 60th day in triple inoculated plants than dual inoculated and control. The results also showed that the soil macronutrients (N, P & K) and micronutrients (Zn, Fe, Cu & Mn) have been found to be maximum at 40th day and 60th day respectively in triple inoculated plants than dual inoculated and control.
Aim: The aim of this study was to evaluate the heavy metal resistance potentiality of endophytic fungi isolated from the leaves of Boswellia ovalifoliolata, an endemic medicinal plant of Tirumala Hills.Methods: Initially, isolation of fungal endophytes was carried out. Isolated fungi were screened for the heavy metal resistance against Co, Cd, Cu and Zn using growth and evaluated their maximum tolerant capacity. Molecular identification of endophytic fungi was carried out by 18S rRNA gene amplification and Sanger's nucleotide sequencing. Phylogenetic tree was constructed using NCBI Clustal W.
Results:Ten different endophytic fungi were isolated from the leaves of B. ovalifoliolata. Among the isolated endophytic fungi, five showed resistance to Co, Cd, Cu, and Zn. The most resistant fungus was identified as Lasiodiplodia theobromae based on 18S rRNA gene sequencing.
Conclusions:L. theobromae was isolated from B. ovalifoliolata and identified as one of the useful fungi involved in mycoremediation against heavy metal toxicity.
The bulk Cd0.8Zn0.2S semiconductor compounds doped with different amounts of magnesium(Mg) have been synthesized by controlled co-precipitation method from aqueous solution containing cadmium acetate, zinc acetate and thiourea at 0.02 mol% of Mgx(x=0~50ml). The solution mixture was made alkaline by adding 25% of liquid ammonia. The structural and electrical conductivity properties of Cd0.8Zn0.2S:Mg samples have been studied using X-Ray Diffraction (XRD) and Electrical Conductivity Studies. The samples have polycrystalline nature with hexagonal structure observed in the XRD studies. The average crystalline size varied from 30 to 47 nm and also calculated lattice parameters. The low temperature electrical conductivity measurements of Cd0.8Zn0.2S: Mg compounds were studied in the range of 77-300K by Keithley electrometer. It is observed that the increase in Mg doping concentration will increases the electrical conductivity. The values of activation energy were estimated for all the samples and found that the maximum activation energy at lower temperature range is 69.27 meV.
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