This paper studies users' learning perception in a virtual titration experiment with differentiated instruction. We develop a virtual reality chemistry lab and use leap motion to detect users' hand gestures for operations. Users wear a head‐mounted display and use their bare hands to interact with virtual objects to perform a titration experiment. Our system implements a complete titration process and provides assistance tools for learning and operating virtual items. We report the essential ideas for building the system. We applied differentiated instruction to study the learning effectiveness of users under different learning intensities. Two groups of students with and without chemistry background participated in a user study. Our results indicated that the virtual reality chemistry lab could enhance and promote users' learning confidence under suitable learning intensities.