This study aimed to investigate the changes that occur in liver function tests in cholelithiasis and relate those changes to it. For this purpose, 38 blood specimens were collected from the patients with cholelithiasis undergoing/underwent Cholecystectomy at Azadi-teaching and Kirkuk hospital from January to June 2021, 19 pre-operative samples, and 19 four weeks post-operatively. Our study showed that the gallstone disease was more common in females 17 (89.46%) than males 2 (10.54%). Age was a major risk factor as the patients’ mean age was 39.26 years. Out of 19 patients undergoing cholecystectomy, pre-operative liver function tests (LFT) were normal in 11(61.11%) patients, and abnormal LFT was detected in 8 (44.44 %) patients. Eight (42.1%) patients had elevated alkaline phosphatase (ALK-P levels%), and also had an elevated of AST and ALT. In the serum, slight elevations in the level of total bilirubin 5 (26.3%), and direct bilirubin 3 (15.8) were also noticed in those patients. Following Cholecystectomy (after 4 weeks), in some cases, changes occur in liver function tests where the finding of ALT, AST, and ALK-P closely shows restoration to normal. However, some cases show no significant variation. Statistically, big distinctions between levels of ALK-P, AST, and ALT have been found before and after Cholecystectomy (p is less than 0.0050, p is less than 0.0124, and p is less than 0.0115). The statistical result shows no significance in total bilirubin (TBIL) and direct bilirubin (DBIL) in postoperative cases. At all, there were big distinctions between levels of ALK-P, AST, and ALT before and after Cholecystectomy (p is less than 0.0050, p is less than 0.0124, and p is less than 0.0115, respectively), but levels of total and direct bilirubin revealed no significant distinctions before and after Cholecystectomy.